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Sodium hypochlorite solution

次氯酸钠溶液

公司名称: Sigma-Aldrich
产品编号: 425044
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Measurement of Intracellular ROS in Caenorhabditis elegans Using 2’,7’-Dichlorodihydrofluorescein Diacetate
Author:
Date:
2018-03-20
[Abstract]   Reactive oxygen species (ROS) are generated during normal metabolic processes under aerobic conditions. Since ROS production initiates harmful radical chain reactions on cellular macromolecules, including lipid peroxidation, DNA mutation, and protein denaturation, it has been implicated in a wide spectrum of diseases such as cancer, cardiovascular disease, ischemia-reperfusion and aging. Over the past several decades, antioxidants have received explosive attention regarding their protective potential against these deleterious reactions. Accordingly, many analytical methodologies have been ... [摘要]  在有氧条件下的正常代谢过程中产生活性氧物质(ROS)。 由于ROS产生会引起细胞大分子的有害自由基连锁反应,包括脂质过氧化反应,DNA突变和蛋白质变性,因此它已涉及多种疾病,如癌症,心血管疾病,缺血再灌注和衰老。 在过去的几十年中,抗氧化剂已经受到爆炸性的关注,因为它们对这些有害反应具有保护作用。 因此,已经开发了许多分析方法用于评估化合物或复杂生物样品的抗氧化能力。 在此,我们介绍了使用2',7'-二氯荧光素二乙酸盐(H 2),在线虫体内用光度计检测体内细胞内ROS水平的简单方便的方法。 DCFDA),一种细胞渗透性示踪剂。

【背景】已经广泛地使用荧光探针2',7'-二氯荧光素二乙酸酯(H 2 DCFDA)原位检测活体内细胞内活性氧簇(ROS)水平由在氧化应激和相关疾病领域工作的研究人员提供。非极性和非离子型探针H 2 DCFDA可以容易地穿透细胞膜并且被酯酶酶促脱乙酰化。该生物化学反应将H 2 DCFDA转化成非荧光化合物H 2 DCF,然后将其迅速氧化成高度荧光的2',7'-二氯荧光素(DCF) ROS的存在(图1A)。因此,来自H 2 ...

Nematode Epicuticle Visualisation by PeakForce Tapping Atomic Force Microscopy
Author:
Date:
2017-11-05
[Abstract]  The free-living soil nematode Caenorhabditis elegans has become an iconic experimental model animal in biology. This transparent animal can be easily imaged using optical microscopy to visualise its organs, tissues, single cells and subcellular events. The epicuticle of C. elegans nematodes has been studied at nanoscale using transmission and scanning (SEM) electron microscopies. As a result, imaging artefacts can appear due to embedding the worms into resins or coating the worms with a conductive gold layer. In addition, fixation and contrasting may also damage the cuticle. ... [摘要]  自由生活的土壤线虫秀丽隐杆线虫已经成为生物学中一个标志性的实验动物模型。这种透明的动物可以很容易地使用光学显微镜成像,以可视化其器官,组织,单细胞和亚细胞事件。 C的角质层。已经使用透射和扫描(SEM)电子显微镜在纳米级研究了线虫线虫。结果,由于将蠕虫嵌入树脂或用导电金层涂覆蠕虫,可能出现成像伪像。另外,固定和对比也可能损伤角质层。传统的敲击模式原子力显微镜(AFM)可用于对干燥的线虫的角质层在空气中成像,然而这种方法也存在成像缺陷。理想情况下,线虫应该在类似自然环境的条件下成像。最近,我们报道了使用PeakForce攻丝AFM模式来成功实现可视化和数值表征。线虫角质层在空气和液体中均有表达(Fakhrullina et al。,2017)。我们成像的主要线虫表面结构和角质层的力学性能表征。该协议提供了对液体浸泡的AF的AFM成像的详细描述。线虫使用PeakForce攻丝原子力显微镜线虫。
【背景】自由生活和寄生虫的线虫由于其对农业和人类福祉的显着生物学效应而受到广泛的研究。显然,线虫中研究最多和最着名的物种是自由生活的土壤线虫秀丽隐杆线虫(Sterken等人,2015年)。这种蠕虫在许多研究中已被成功地用作多功能模式生物体(Fire等人,1998; Kenyon,2010; Swierczek等人,2011; O'Reilly等人,2014; ...

Development and Implementation of an in vitro Culture System for Intact Detached Grape Berries
Author:
Date:
2015-06-20
[Abstract]  Grape composition depends on the metabolites accumulated and synthesized during grape development. It is of paramount importance for grape growers because of its major role in shaping wine quality. Therefore, understanding the regulation mechanisms that control the accumulation of quality-related metabolites in grape is of both scientific and agronomical interests. The composition of grape berry at harvest is under complex regulation and can be affected by many factors (Conde et al., 2007). The study of the effects of these factors on berries still attached to intact plants can be ... [摘要]  葡萄组成取决于葡萄发育过程中积累和合成的代谢物。这对于葡萄种植者是至关重要的,因为它在塑造葡萄酒品质方面起着重要作用。因此,理解控制质量相关代谢物在葡萄中的积累的调节机制具有科学和农业利益。收获时葡萄浆的组成受到复杂调节,并且可能受到许多因素的影响(Conde等人,2007)。这些因素对仍然附着于完整植物的浆果的影响的研究可能是高度挑战性的,因为植物的大尺寸,植物间,群集间和互花间变异性;并且因为精确地控制由浆果和环境进入的营养物和激素是复杂的。因此,体外培养的葡萄浆果是一种良好的模型系统,其比葡萄细胞悬浮液更好地代表浆果解剖结构(皮肤和肉),并且与整株植物相比大大降低了系统复杂性(Bravdo et al。,1990;Pérezet al。,2000; Gambetta et al。,2010)。为此,通过将温室果枝切割生产和体外器官培养技术相结合,开发了完整脱离的葡萄浆果的体外培养系统(Dai等人al。,2014)。培养的浆果能够积极地吸收和利用来自培养基的碳和氮,并且表现出果实成熟特征,例如变色和软化。这种体外系统可用于研究浆果组合物对环境和营养因素的响应。

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