{{'Search' | translate}}
 

Glycerol

甘油

公司名称: Sigma-Aldrich
产品编号: G5516
Bio-protocol()
Company-protocol()
Other protocol()

Purification of Rice Stripe Virus
Author:
Date:
2020-03-20
[Abstract]  Although many spherical and rod-shaped plant virus purification protocols are now available, only a few protocols on filamentous plant virus purification have been published. Here, we report a protocol for large-scale purification of Rice stripe virus (RSV) from RSV-infected rice tissues. RSV virions with high infectivity were first precipitated with polyethylene glycol (PEG) followed by pelleting through primary ultracentrifugation, ultracentrifugation in a glycerol cushion and ultracentrifugation in density gradient. The purified RSV virions can not only be viewed as filamentous particles ... [摘要]  [摘要 ] 尽管现在可以使用许多球形和杆状植物病毒的纯化方案,但有关丝状植物病毒纯化的协议却只有少数。在这里,我们提出一个协议水稻的大规模纯化小号牛肚v IRUS 从RSV感染的水稻组织(RSV)。首先用聚乙二醇(PEG)沉淀具有高感染力的RSV 病毒颗粒,然后通过一次超速离心,在甘油垫层中超速离心和密度梯度超速离心沉淀。纯化的RSV 病毒体不仅可以在电子显微镜下观察为丝状颗粒,而且还可以通过昆虫载体直接注入昆虫体内或在送入水稻之前通过膜饲喂获得。

[背景 ] 许多purificatio n,用于球形和棒状病毒协议已被发表(安德烈é 等人,2002; BALKE 。等人,2018) 。这些协议都依赖于化学沉淀或密度梯度离心。但是,丝状病毒的纯化方案目前受到限制。

大米小号肚v 病毒属(RSV)是负链RNA病毒,属于属纤细病毒,订单Bunyavirales 。在许多东亚国家,RSV经常对水稻生产造成严重损害(Whitfield 等,2015; Liu 等,2018)。与生产球状和包膜病毒体的Bunyavirales 顺序中的其他成员不同,RSV 病毒体是丝状的。然而,RSV基因组编码在纯化的RSV 病毒体中未发现的糖蛋白(Toriyama,19 86; Lu 等人,2019)。已知RSV ...

Manganese Superoxide Dismutase Activity Assay in the Yeast Saccharomyces cerevisiae
Author:
Date:
2020-03-05
[Abstract]  Superoxide dismutases (SODs) act as a primary defence against reactive oxygen species (ROS) by converting superoxide anion radicals (O2-) into molecular oxygen (O2) and hydrogen peroxide (H2O2). Members of this enzyme family include CuZnSODs, MnSODs, FeSODs, and NiSODs, depending on the nature of the cofactor that is required for proper activity. Most eukaryotes, including yeast, possess CuZnSOD and MnSOD. This protocol aims at assessing the activity of the yeast Saccharomyces cerevisiae MnSOD Sod2p from cellular extracts using ... [摘要]  [摘要 ] 超氧化物歧化酶(SOD能)充当主防御针对反应性氧物质(ROS)通过转换的超氧阴离子自由基(O 2 - )为分子氧(O 2 )和过氧化氢(H 2 ? 2 )。这种酶的家庭成员包括CuZnSODs ,MnSODs ,FeSODs 和NiSODs ,这取决于是需要适当的活动辅助因子的性质。大多数真核生物,包括酵母,都具有CuZnSOD 和MnSOD 。该协议旨在评估酵母的活性 使用硝基蓝四唑染色法从细胞提取物中提取酿酒酵母MnSOD Sod2p 。该方法可用于估计Mn 2+ 的细胞生物利用度以及评估细胞的氧化还原状态。

[背景 ] 的SODs被定义为减少正常有氧代谢为氧气和过氧化氢期间形成的氧的有害自由基含金属的抗氧化剂酶。:这些酶是基于需要作为辅因子进行适当的酶活性的金属分类CuZnSODs ,MnSODs ,FeSODs ,和NiSODs 。在酿酒酵母中,有两个S OD :CuZn-Sod1p和Mn-Sod2p(Abreu和Cabelli ...

Purification of Soluble Recombinant Human Tau Protein from Bacteria Using Double-tag Affinity Purification
Author:
Date:
2018-11-20
[Abstract]  Dysfunction of the microtubule-associated protein Tau (encoded by the MAPT gene) has been implicated in more than twenty neurodegenerative diseases, including Alzheimer’s. As such, the physiological and disease-relevant functions of Tau have garnered great interest in the research community. One barrier hampering investigations into the functions of Tau and the generation of pharmacological agents targeting Tau has been the difficulty of obtaining soluble Tau protein in purified form. Here, we describe a protocol that uses dual affinity tag purification to selectively purify soluble ... [摘要]  微管相关蛋白Tau(由 MAPT 基因编码)的功能障碍已经涉及20多种神经退行性疾病,包括阿尔茨海默病。 因此,Tau的生理和疾病相关功能引起了研究界的极大兴趣。 妨碍对Tau功能的研究和产生靶向Tau的药理学试剂的一个障碍是难以获得纯化形式的可溶性Tau蛋白。 在这里,我们描述了一种方案,该方案使用双亲和标签纯化从细菌中选择性纯化可溶性重组Tau蛋白,所述细菌对于下游应用具有功能活性,包括免疫,微管结合测定和蛋白质 - 蛋白质相互作用研究。
【背景】Tau传统上被定义为微管结合蛋白;然而,在人类疾病中,Tau可以与轴突微管分离并错误定位到其他神经元区室,包括体细胞,树突和突触,其中与非微管蛋白和结构的相互作用驱动神经元功能障碍(Iqbal et al。 ,2016; Wang和Mandelkow,2016; Zhou et al。,2017; McInnes et al。,2018)。尽管神经原纤维缠结形式的Tau聚集体通常存在于死后患病的脑组织中,但研究表明,可溶性Tau,而不是聚集的Tau,是神经元功能障碍的主要原因(Crimins et al。,2012 ; Polydoro et al。,2014; Koss et al。,2016)。因此,研究Tau在疾病中的可溶性功能,例如鉴定蛋白质 - ...

产品评论