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Similar Protocol
A Pulse–chase EdU Method for Detection of Cell Division Orientation in Arabidopsis and
Juncus prismatocarpus
Leaf Primordia
Xiaofeng Yin
and
Hirokazu Tsukaya
Published online: 2021-01-05
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Pollen Germination and Pollen Tube Growth of
Arabidopsis thaliana
:
In vitro
and Semi
in vivo
Methods
Author:
Hugh Dickinson
,
Josefina Rodriguez-Enriquez
and
Robert Grant-Downton
,
Date:
2018-08-20
[Abstract] Studies of pollen germination and post-germination development are not only essential for understanding plant reproduction but also are an excellent model system for tip-based growth. Here we describe easy, reproducible methods for germination and growth of pollen from the model plant
Arabidopsis thaliana
in artificial conditions. Our growth system can be used both for pollen placed directly on this artificial substrate as well as for the so-called ‘semi
in vivo
’ method. This is where a pistil is cut shortly after hand-pollination and the pollen tubes grow through the plant ...
[摘要