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公司名称: GE Healthcare
产品编号: 28-4013-53
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Probing Yeast Protein Microarrays for Protein-protein Interactions Using V5-epitope Tagged Fusion Protein Probes
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Date:
2012-03-05
[Abstract]  Protein microarray is probably the only technique currently available for systematic investigation of protein-protein interactions. This protocol describes an optimized method to probe yeast protein microarrays for protein-protein interactions using purified V5-epitope tagged fusion protein. It should also apply to any other proteins with appropriate modifications. [摘要]  目前,蛋白芯片可能是唯一一种验证蛋白之间相互作用的有效的工具。这种方法是通过用V5-抗原表位标签的融合蛋白标记酵母蛋白芯片,探究蛋白之间的相互作用。同时,这种方法也可以应用于其它任何一个修饰的蛋白。

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