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Horse Serum, heat inactivated, New Zealand origin

马血清,热灭活,新西兰来源

公司名称: Thermo Fisher Scientific
产品编号: 26050088
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Isolation, Culturing, and Differentiation of Primary Myoblasts from Skeletal Muscle of Adult Mice
Author:
Date:
2017-05-05
[Abstract]  Myogenesis is a multi-step process that leads to the formation of skeletal muscle during embryonic development and repair of injured myofibers. In this process, myoblasts are the main effector cell type which fuse with each other or to injured myofibers leading to the formation of new myofibers or regeneration of skeletal muscle in adults. Many steps of myogenesis can be recapitulated through in vitro differentiation of myoblasts into myotubes. Most laboratories use immortalized myogenic cells lines that also differentiate into myotubes. Although these cell lines have been found ... [摘要]  造血是一种多步骤过程,导致在损伤的肌纤维的胚胎发育和修复期间骨骼肌的形成。在这个过程中,成肌细胞是主要的效应细胞类型,彼此融合或损伤肌纤维,导致新成肌纤维的形成或成年人骨骼肌的再生。通过体外成骨细胞分化成肌管可以概括出许多发生肌肉发育的步骤。大多数实验室使用也分化成肌管的永生化肌原细胞系。虽然已经发现这些细胞系对于描绘造血的调节机制非常有用,但是它们通常依赖于细胞的来源和培养条件而显示出很大的变异性。原代成肌细胞被认为是体外研究肌生成的最生理学相关模型。然而,由于成体骨骼肌的丰度低,原代成肌细胞的分离在技术上是有挑战性的。在本文中,我们描述了一种用于从小鼠的成年骨骼肌分离原代成肌细胞的改进方案。我们还描述了其培养和分化成肌管的方法。


背景 造血是一个复杂而高度协调的过程,其涉及多潜能中胚层细胞的测定,以产生成肌细胞,成肌细胞从细胞周期中排出,以及它们最终分化为骨骼肌纤维。 Myogen-5,MyoD,myogenin和MRF4的基因螺旋 - 环 - 螺旋转录因子的一组基因调控因子(MRFs)的顺序表达调控。 Myf-5和MyoD是成肌细胞形成,增殖和存活所需的主要MRFs,而其他MRF(如肌细胞生成素和MRF-4)在肌发生过程中起作用迟发,激活收缩蛋白和其他结构和代谢蛋白的基因表达(白金汉,2003; ...

Heterochronic Pellet Assay to Test Cell-cell Communication in the Mouse Retina
Author:
Date:
2017-02-05
[Abstract]  All seven retinal cell types that make up the mature retina are generated from a common, multipotent pool of retinal progenitor cells (RPCs) (Wallace, 2011). One way that RPCs know when sufficient numbers of particular cell-types have been generated is through negative feedback signals, which are emitted by differentiated cells and must reach threshold levels to block additional differentiation of that cell type. A key assay to assess whether negative feedback signals are emitted by differentiated cells is a heterochronic pellet assay in which early stage RPCs are dissociated and labeled with ... [摘要]  构成成熟视网膜的所有七种视网膜细胞类型都是由普通的多能视网膜祖细胞池(RPC)产生的(Wallace,2011)。已经产生足够数量的特定细胞类型的RPC知道的一种方式是通过负反馈信号,其由分化细胞发射并且必须达到阈值水平以阻止该细胞类型的额外分化。评估负反馈信号是否由分化细胞发出的关键测定是异源沉淀测定,其中早期RPC被解离并用BrdU标记,然后与20倍过量的解离的分化细胞混合。然后将组合的细胞再次聚集并在细胞膜上培养7-10天。在这段时间内,RPC将会分化,BrdU + RPC的命运可以使用细胞类型特异性标记进行评估。开发这种沉淀测定的研究人员最初表明,当两种细胞类型混合在一起时,新生儿RPC与胚胎RPC相比,在加速进度条件下产生杆(Watanabe和Raff,1990; Watanabe等,1997)。我们已经使用这种测定来证明我们发现作为视网膜神经节细胞(RGC)分化的负调节物的声刺猬(Shh)促进RPC增殖(Jensen和Wallace,1997; Ringuette等,2014)。最近我们修改了异质性沉淀测定法,以评估视网膜无长突细胞的反馈信号的作用,将转化生长因子β2(Tgfβ2)鉴定为负反馈信号,并将Pten作为Tgfβ2应答的调节剂(Ma et al。,2007 ; ...

Skeletal Myogenesis in vitro
Author:
Date:
2015-11-05
[Abstract]  Mature skeletal myofibers are elongated and multinucleated cells. Many stem/progenitor cell types, including committed muscle stem (satellite cells) and progenitor (myoblasts) cells, muscle-derived stem cells, myogenic endothelial cells, and mesenchymal stem/stromal cells, have been shown to exhibit skeletal myogenesis under appropriate inductive conditions. Committed muscle stem/progenitor cells and multipotent stem/progenitor cells which have skeletal myogenic capacity can typically be differentiated into skeletal myofibers in vitro following extended low-serum exposure. ... [摘要]  成熟骨骼肌纤维是细长和多核细胞。 许多干/祖细胞类型,包括定型肌干(卫星细胞)和祖细胞(成肌细胞)细胞,肌肉衍生的干细胞,肌肉内皮细胞和间充质干/基质细胞,已显示在适当的诱导条件下表现出骨骼肌发生 。 具有骨骼肌生成能力的承诺的肌肉干/祖细胞和多能干/祖细胞可以在延长的低血清暴露后通常在体外分化成骨骼肌纤维。 分化的细胞表现出不同的纤维样细长形态与多个核,并表示独特的肌肉分子标记指示肌发生,包括结蛋白(早)和快和/或慢肌球蛋白重链(成熟)。

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