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Salicylhydroxamic acid

水杨酸异羟肟酸

公司名称: Sigma-Aldrich
产品编号: S607
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Measurement of Mitochondrial Respiration Rate in Maize (Zea mays) Leaves
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2015-05-20
[Abstract]  Mitochondria play essential roles in plant growth and development as they host the oxidative phosphorylation pathways, tricarboxylic acid cycle and other important metabolisms. Disruption of mitochondrial functions frequently leads to embryo lethality. Moreover, mitochondria play roles in programmed cell death, pathogen and stress responses in plants. In contrast to animal mitochondria, plant mitochondria possess an additional electron transport pathway, the cyanide-resistant alternative pathway catalyzed by a single alternative oxidase (AOX). Unlike cytochrome pathway that is coupled to ... [摘要]  线粒体在植物生长和发育中起重要作用,因为它们宿主氧化磷酸化途径,三羧酸循环和其他重要代谢。线粒体功能的破坏经常导致胚胎致死。此外,线粒体在程序性细胞死亡,病原体和植物应激反应中发挥作用。与动物线粒体相反,植物线粒体具有额外的电子传递途径,由单一备选氧化酶(AOX)催化的氰化物抗性替代途径。与通过质子易位与氧化磷酸化偶联的细胞色素途径不同,从泛素到AOX的电子转运是非磷酸化的。它释放能量作为热。 Chlorolab II液相氧电极(Hansatech)是一种高精度的Clark型氧电极,配有强大的WINDOWS软件,可以实时记录氧变化。其电极盘包括中心铂阴极和同心银阳极。电极盘连接到在铂和银电极之间施加小的极化电压的电极控制单元。在氧的存在下,产生与样品中的氧含量成比例的小电流。它可以敏感地和快速地对样品中氧含量的小变化作出响应。该协议描述如何测量线粒体总呼吸率,细胞色素通路能力以及具有chlorolab II氧电极的玉米叶中的替代途径能力。

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