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DMEM/F-12, HEPES

DMEM / F-12,HEPES

公司名称: Thermo Fisher Scientific
产品编号: 11330032
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Isolation and Primary Culture of Adult Human Adipose-derived Stromal/Stem Cells
Author:
Date:
2017-03-05
[Abstract]  Adipose-derived stromal/stem cells (ASCs) are multipotent cells that can be isolated from adipose tissue. Studies have shown that cells have the capacity to self-renew and differentiate into adipocyte, chondrocyte, myocyte, and osteoblast lineages. Thus, significant interest regarding their use for regenerative purposes to restore aging or damaged tissue has grown in recent decades. These cells have also been shown to immunomodulate the microenvironment and secrete abundant growth factors, which minimize inflammation and aid repair and regeneration. ASCs can be readily isolated from the ... [摘要]  脂肪来源的基质/干细胞(ASCs)是可以从脂肪组织分离的多能细胞。研究表明,细胞具有自我更新和分化成脂肪细胞,软骨细胞,肌细胞和成骨细胞谱系的能力。因此,近几十年来,对再生用途恢复老化或损伤组织的兴趣越来越大。这些细胞也被证明可以免疫微环境并分泌丰富的生长因子,从而使炎症最小化并辅助修复和再生。 ASCs可以容易地从脂质体的基质血管分数(SVF)中分离出来。鉴于其易于获取,丰富的来源和在再生医学和组织工程中的潜在应用,对于ASC的表征和利用越来越感兴趣。该方案描述了从成人人类脂肪组织中分离的ASC以及用于培养维持的方法,包括扩增和低温保存。

背景 脂肪来源的基质/干细胞(ASCs)表现出干细胞领域的巨大潜力。根据造血干细胞移植的治疗奇迹,ASCs代表干细胞的未来,因为它们更容易获得源 - 脂肪组织。 ASCs自我更新和分化成各种组织谱系(包括脂肪细胞,软骨细胞,肌细胞和成骨细胞谱系)的能力允许恢复损伤的组织。另外,推测ASCs有可能在体外复制组织。器官将允许更容易获得新颖药物的评估,从而显着降低药物生产成本。然而,隔离,维护和冷冻保存过程中的不一致,禁止集体分析世界各地不同实验室的结果。用于分离和培养ASC的标准方案对于确保一致的数据分析是必要的。

Isolation and Culture of Human Adipose-derived Stem Cells from Subcutaneous and Visceral White Adipose Tissue Compartments
Author:
Date:
2016-11-20
[Abstract]  Human Adipose-derived Stem/Stromal Cells (ASCs) have been widely used in stem cell and obesity research, as well as clinical applications including cell-based therapies, tissue engineering and reconstruction. Compared with mesenchymal stem cells (MSCs) derived from other tissues such as umbilical cord and bone marrow, isolation of ASCs from human white adipose tissue (WAT) has great advantages due to its rich tissue source and simple surgical procedure. In this detailed protocol we describe a protocol to isolate and characterize ASCs from human WAT. Molecular characterization of isolated ASCs ... [摘要]  Human Adipose-derived Stem/Stromal Cells (ASCs) have been widely used in stem cell and obesity research, as well as clinical applications including cell-based therapies, tissue engineering and reconstruction. Compared with mesenchymal stem cells (MSCs) derived from other tissues such as umbilical cord and bone marrow, isolation of ASCs from human white adipose tissue (WAT) has great advantages due to its rich tissue source and simple surgical procedure. In this detailed protocol we describe a protocol to isolate and characterize ASCs from human WAT. Molecular characterization of isolated ASCs ...

Neurosphere Co-culture Assay
Author:
Date:
2016-08-05
[Abstract]  The hippocampal niche is one of two areas in the brain where stem cells reside. In this neurogenic niche, stem cells can be found in close proximity to astrocytes and in contact with microvessels consisting of pericytes and endothelial cells. To study the regulatory interplay of this complex niche network in a simplified in vitro model, we established a co-culture system. We investigate the formation of neurosphere under different co-culture conditions by using primary niche cells. Here, we describe the isolation procedure for primary niche cells culture of astrocytes, endothelial ... [摘要]  真菌形态发生需要细胞壁的修饰和可塑性,这意味着其组分(包括壳多糖和葡聚糖)的合成和重塑。因此几丁质酶和葡聚糖酶活性对于细胞壁生物发生和细胞分裂是至关重要的。几丁质酶活性的定量可能有助于鉴定可能负面影响一些丝状真菌如产生细胞内和分泌型几丁质酶的尖孢镰刀菌的生长和形态发生的结构缺陷。基于它们对壳多糖底物的酶作用将壳聚糖分解酶分类。内酯酶被定义为催化几丁质链中内部点的随机裂解的酶。外切球蛋白酶催化乙酰壳寡糖或N-乙酰葡糖胺从几丁质的非还原端逐步释放,因此分别称为壳聚糖酶和β-N-乙酰氨基葡糖苷酶。在这里,我们描述了一种简单的方法来轻易地纯化几丁质酶,以便比较不同的F的内切几丁质酶活性和外切几丁质酶活性。尖孢镰孢菌株。该方案可适用于任何真菌物种。...

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