{{'Search' | translate}}
 

Neurobasal® Medium, minus phenol red

Neurobasal ®培养基,不含酚红

公司名称: Thermo Fisher Scientific
产品编号: 12348017
Bio-protocol()
Company-protocol()
Other protocol()

Generation of Neuron-enriched Cultures (Method 2)
Author:
Date:
2011-11-05
[Abstract]  Neuron-enriched cultures are a useful tool to study neuronal development and the molecular pathways that come in to play during neuronal death in various neurological disorders. This protocol is for generating midbrain neuronal cultures from late embryo rodent brain. This method uses Neurobasal medium and B27 serum-free supplement. The long-lasting (> 4 weeks) midbrain neuron-enriched cultures generated following this protocol have been wildly used to study the pathogenesis of Parkinson’s disease, the most common neurodegenerative movement disorder. The neuron-enriched cultures prepared ... [摘要]  神经元富集培养是来研究在神经元死亡中各种神经系统疾病的分子通路的有用工具。本方法可用于从啮齿动物后期胚胎大脑获得中脑神经元培养。本方法使用神经基质培养基以及B27无血清补充物。 本实验方法所获得的长期(大于四周)中脑神经元富集培养已广泛用于研究帕金森氏病这一最常见的神经退化性运动障碍疾病的发病机制。本方法准备的神经元富集将含有<10%的星形胶质细胞。使用b27无血清补充物将肯定会增加成本。此实验方法经dr. hong实验室不同研究人员多年的发展和完善,尤其是dr. bin liu. hong实验室不同研究人员多年的发展和完善,尤其是dr.="" bin="">

Generation of Neuron-enriched Cultures (Method 1)
Author:
Date:
2011-11-05
[Abstract]  This protocol will guide you through the process for generating midbrain neuronal cultures from late embryo mouse brain. These cultures serve as a useful tool to study molecular pathways during neuronal death in various neurological disorders. This method uses cytosine β-D-arabinofuranoside in the cultures to suppress the proliferation of glial cells. Seven days after the seeding, the neuron-enriched cultures prepared following this protocol will contain less than 10% glia cultures. The concentrations of β-D-arabinofuranoside should be adjusted according to your culture condition. This ... [摘要]  本方法将引导您完成从后期胚胎小鼠大脑产生中脑神经元培养的整个过程。这种培养是来研究在神经元死亡中各种神经系统疾病的分子通路的有用工具。这种方法使用胞嘧啶β-D-arabinofuranoside(阿糖胞嘧啶)到培养基中,抑制胶质细胞增生。播种后第7天,在本方法后的神经元富集的准备将含有不少于10%的神经胶质细胞培养基。β-D-arabinofuranoside的浓度应根据培养基条件调整。此实验方法经Dr. Hong实验室不同研究人员多年的发展和完善,尤其是Dr. Bin Liu.

产品评论