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CHAPS hydrate

CHAPS水合物

公司名称: Sigma-Aldrich
产品编号: C9426
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A Phosphopeptide Purification Protocol for the Moss Physcomitrella paten
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2015-07-20
[Abstract]  Protein phosphorylation is one of the most common post-translational modifications in eukaryotic cells and plays a critical role in a vast array of cellular processes. Efficient methods of protein extraction and phosphopeptide purification are required to ensure the detection of high quality of proteins. In our hands, phenol extraction of proteins and TiO2 chromatography enrich phosphorylated peptides more efficiently than other methods in the moss Physcomitrlla patens (P. patens). [摘要]  蛋白磷酸化是真核细胞中最常见的翻译后修饰之一,并在大量细胞过程中起关键作用。 需要蛋白质提取和磷酸肽纯化的有效方法以确保高质量的蛋白质的检测。 在我们的手中,蛋白质和TiO 2层析的酚提取比在小麦Physcomitrlla patens中的其它方法更有效地富集磷酸化肽。 )。

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