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Formalin

10%福尔马林溶液,中性缓冲液

公司名称: Sigma-Aldrich
产品编号: HT5014
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Immunohistochemistry of Kidney a-SMA, Collagen 1, and Collagen 3, in A Novel Mouse Model of Reno-cardiac Syndrome
Author:
Date:
2020-09-20
[Abstract]  Cardiorenal syndrome defines a synergistic pathology of the heart and kidneys where failure of one organ causes failure in the other. The incidence of cardiovascular mortality caused by this syndrome, is 20 fold higher in the end stage renal disease (ESRD) population compared to the population as a whole thus necessitating the need for improved therapeutic strategies to combat reno-cardiac pathologies.

Murine in vivo models play a major role in such research permitting precise genetic modification thus reducing miscellany, however presently there is no steadfast model of ...
[摘要]  [摘要 ] 心肾综合征定义了心脏和肾脏的协同病理,其中一个器官的衰竭导致另一个器官的衰竭。与整个人群相比,该综合征导致的心血管疾病死亡率在终末期肾脏病(ESRD)人群中要高出20倍,因此有必要改善治疗策略以应对肾病。

小鼠体内模型在允许精确基因修饰从而减少杂项的研究中起主要作用,但是目前在最常见的基因修饰小鼠品系C57BL / 6小鼠中还没有稳定的雷诺-心脏综合征模型。在这项研究中,我们使用腺嘌呤饮食修改了已建立的慢性肾脏疾病模型,并扩展了在C57BL / 6小鼠中实现慢性肾功能衰竭和随之而来的肾心脏综合征的相关病理。

使八周大的雄性C57BL / 6小鼠适应7天,然后给予0.15%腺嘌呤饮食或对照饮食20周,此后终止实验,收集血液,尿液和器官并进行生化和免疫组织化学分析。

施用0.15%的腺嘌呤饮食会导致进行性肾功能衰竭,从而导致肾性心脏病综合征,这可通过心体重比显着增加来证实(P <0.0001)。血液生化表明,用腺嘌呤喂养的小鼠血清肌酐,尿素含量显着增加(P <0.0001),肾小球滤过率显着降低(P <0.05),而肾脏的α-SMA,胶原蛋白1和胶原蛋白3免疫组化显示严重的纤维化。

我们提出了一种新型的腺嘌呤饮食方案,该方案在C57BL / ...

Mouse Model of Immune Complex-mediated Vasculitis in Dorsal Skin and Assessment of the Neutrophil-mediated Tissue Damage
Author:
Date:
2017-12-20
[Abstract]  Neutrophils are the most abundant leukocytes in the blood. In the recent decades, their crucial roles in host defense, immune regulation and tissue damage have been studied in a deeper dimension. In this protocol, we described a mouse model of immune complex-mediated vasculitis in the dorsal skin induced by Arthus reaction, and the subsequent analysis of edema, hemorrhage and tissue damage due to neutrophil activation by means of Evans blue area analysis, histology, and immunofluorescence. This protocol could facilitate the investigation of cellular therapy strategy against over-activated ... [摘要]  嗜中性粒细胞是血液中最丰富的白细胞。 近几十年来,它们在宿主防御,免疫调节和组织损伤方面的关键作用已经得到了更深入的研究。 在这个协议中,我们描述了Arthus反应诱导的背部皮肤中的免疫复合物介导的血管炎的小鼠模型,以及随后通过伊文思蓝区域分析,组织学和分析由于嗜中性粒细胞活化引起的水肿,出血和组织损伤免疫荧光。 该协议可以促进细胞治疗策略对过度活化的嗜中性粒细胞介导的组织损伤的调查。

【背景】嗜中性粒细胞构成循环白细胞的最大的进化保守部分。他们引导第一波主机防御感染或组织损伤。嗜中性粒细胞介导的细胞毒性的体外模型已被充分证实(Incani等人,1981; Dallegri等人,1984; Saffarzadeh等人,等人,2012年)。但是,为了剖析中性粒细胞介导的无菌组织损伤的复杂性,“体内”模型是必不可少的。

免疫复合物(IC)介导的血管炎是由抗原 - 抗体复合物在血管中沉积引发的疾病,其随后导致补体激活,嗜中性粒细胞募集和活化。活化的嗜中性粒细胞释放的大量活性氧和蛋白酶损害血管壁的内皮衬里并导致水肿和出血(Sindrilaru等人,2007; Goerge等人, ,2008; ...

Alcian Blue – Alizarin Red Staining of Mouse Skeleton
Author:
Date:
2012-04-20
[Abstract]  Our lab has used the Alcian blue – Alizarin red staining method with certain modifications to characterize skeleton deformities in mice lacking Pek/Perk, encoding a translational control eIF2alpha kinase. [摘要]  我们的实验室已使用经一些改进的阿尔辛蓝 - 茜素红染色法[1]来描绘因缺乏Pek/Perk的小鼠骨骼畸形。这个基因编码了调控翻译的eIF2α激酶 [2]。

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