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Greiner centrifuge tubes

Greiner离心管

公司名称: Sigma-Aldrich
产品编号: T2318
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Heterologous Expression and Purification of the CRISPR-Cas12a/Cpf1 Protein
Author:
Date:
2018-05-05
[Abstract]  This protocol provides step by step instructions (Figure 1) for heterologous expression of Francisella novicida Cas12a (previously known as Cpf1) in Escherichia coli. It additionally includes a protocol for high-purity purification and briefly describes how activity assays can be performed. These protocols can also be used for purification of other Cas12a homologs and the purified proteins can be used for subsequent genome editing experiments.


Figure 1. Timeline of activities for the ...
[摘要]  该协议提供了分步说明(图1),用于在大肠杆菌中异源表达新西兰弗朗西斯菌弗朗西丝菌Cas12a(以前称为Cpf1)。 它还包括一个高纯度纯化方案,并简要介绍如何进行活性测定。 这些方案也可以用于其他Cas12a同系物的纯化,并且纯化的蛋白质可以用于随后的基因组编辑实验。

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图1.从大肠杆菌 异源表达和纯化<弗朗西斯弗朗西丝菌 Cas12a(FnCas12a)的活动时间表

【背景】原核CRISPR-Cas免疫系统通过使用CRISPR RNA(crRNA)作为外源DNA或RNA的序列特异性靶向的指导来提供针对病毒和质粒的保护(van der Oost等人,2014; Marraffini ,2015)。 1类CRISPR-Cas系统(包含I型,III型和IV型)通常形成多亚基蛋白-cRNA效应复合物,而2类系统(包含II型,V型和VI型)依赖于单个crRNA-引导的效应物核酸酶用于目标干扰(Mohanraju et al。 2016年)。

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Aspergillus terreus Infection of Fruits and Terrein Quantification by HPLC Analysis
Author:
Date:
2016-06-20
[Abstract]  The opportunistic fungal human and plant pathogen Aspergillus terreus (A. terreus) can be isolated from sea water, soil or decaying organic matter such as rotting leaves and fruits. While growing on fruits A. terreus produces secondary metabolites such as terrein, which may ease its penetration into plant tissues. In addition, biological activities of terrein may support competition against other microorganisms. In summary, terrein is a small polyketide that reduces germination of seedlings, induces lesions on fruit surfaces but also shows moderate antifungal ... [摘要]  机体真菌人和植物病原菌土曲霉(A. terreus)可以从海水,土壤或腐烂的有机物质如腐烂的叶子和果实中分离出来。 当在水果上生长时,A. terreus产生次生代谢物如土曲杆菌,这可以缓解其渗透到植物组织中。 此外,土耳特蛋白的生物活性可能支持与其他微生物的竞争。 总而言之,土豆蛋白是一种减少幼苗发芽的小聚酮,诱发水果表面的病变,但也显示出中等的抗真菌活性。 使用这份手稿,我们提供了一种水果感染方案与土曲霉,随后使用基于HPLC的量化方法测定感染果实的土豆油生产率。

Ustilago maydis Virulence Assays in Maize
Author:
Date:
2016-03-20
[Abstract]  The basidiomycetous smut fungus Ustilago maydis (U. maydis) infects all aerial parts of its host plant maize (Zea mays L.). Infection symptoms are seen in the form of prominent tumors on all aerial parts of maize, after the establishment of a biotrophic interaction with the host usually around 5-6 days post infection (dpi). The fungus colonizes the various developmentally distinct aerial organs at different stages of development to form these prominent symptoms. Although being a biotrophic plant pathogen, U. maydis can easily be cultivated under axenic ... [摘要]  落叶青霉菌(Basidiomycetous smut fungus)Ustilago maydis( U。maydis )感染其宿主植物玉米的所有地上部分( Zea mays 感染症状在与宿主生物营养相互作用通常在感染后约5-6天(dpi)建立后以玉米的所有地上部分的突出肿瘤的形式见到。真菌在不同发育阶段定居各种发育不同的气生器官以形成这些突出的症状。虽然是一种生物营养性植物病原体, maydis 可以容易地在无菌条件下培养以产生标准化接种物。感染可以在实验室条件下通过注射器接种在玉米的所有气生器官上进行。该方案已经成功地用于感染玉米的所有气生器官并且在U中制定毒力测定。 maydis 使其成为研究植物病理学研究的优良模型系统(Schilling等人,2014; Redkar等人,2015)。

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