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β-Glycerophosphate disodium salt hydrate

β-甘油磷酸二钠盐水合物

公司名称: Sigma-Aldrich
产品编号: G9891
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Binding to Secreted Bone Matrix in vitro
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2014-02-20
[Abstract]  This method examines the bone matrix binding capacity of proteins. Using osteogenic differentiation medium, multipotent stromal cells (MSC) are induced to differentiate into osteocytes in vitro and to secrete bone matrix. The latter is confirmed using Alizarin red S staining, which detects the presence of calcific deposits (hydroxyapatite). These calcific deposits are used to test the bone binding properties of proteins. The binding to the calcific deposits is assessed by Western blot analysis. [摘要]  这种方法检查蛋白质的骨基质结合能力。 使用成骨分化培养基,诱导多潜能基质细胞(MSC)在体外分化成骨细胞和分泌骨基质。 后者使用茜素红S染色证实,其检测钙化沉积物(羟基磷灰石)的存在。 这些钙沉积物用于测试蛋白质的骨结合性质。 通过蛋白质印迹分析评估与钙化沉积物的结合。

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