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UltraPureTM Ethylenediaminetetraacetic Acid, Disodium Salt, Dihydrate (Na2EDTA•2H2O)

UltraPureTM乙二胺四乙酸,二钠盐,二水合物

公司名称: Thermo Fisher Scientific
产品编号: 15576028
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Cell Synchronization by Double Thymidine Block
Author:
Date:
2018-09-05
[Abstract]  Cell synchronization is widely used in studying mechanisms involves in regulation of cell cycle progression. Through synchronization, cells at distinct cell cycle stage could be obtained. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication. Here, we present the protocol to synchronize cells at G1/S boundary by using double thymidine block. After release into normal medium, cell population at distinct cell cycle phase could be collected at different time points. [摘要]  细胞同步广泛用于研究涉及细胞周期进程调节的机制。 通过同步,可以获得不同细胞周期阶段的细胞。 胸苷是一种DNA合成抑制剂,可在DNA复制前阻止细胞在G1 / S边界。 在这里,我们提出了使用双胸苷阻滞同步G1 / S边界细胞的协议。 释放到正常培养基中后,可以在不同的时间点收集不同细胞周期阶段的细胞群。

【背景】细胞周期和细胞分裂是细胞生物学的核心。为了构建多细胞生物体,细胞复制对于产生可以执行特定功能的特化细胞是必需的。正常细胞周期由间期(G1期,S期和G2期)和有丝分裂期(M期)组成(Rodríguez-Ubreva et al。,2010;Léger et al。 ,2016)。在间期期间,遗传物质被复制并使一切为有丝分裂做好准备。然而,在有丝分裂期,重复的染色体被分离并分配到子细胞中(Sakaue-Sawano 等人,,2008)。

为了精确保存遗传信息,必须严格控制细胞周期进程。细胞周期蛋白/ CDK复合物通过在各自阶段快速促进活动来控制细胞周期进展,并且当它们的阶段完成时迅速失活(Graña和Reddy,1995)。

细胞同步对于研究细胞周期调节事件特别有用。使用不同的方法,细胞可以在不同的细胞周期阶段同步。治疗作为微管形成抑制剂的诺考达唑可以使细胞处于G2 / M期同步(Ho et ...

Whole-mount Enteroid Proliferation Staining
Author:
Date:
2016-06-20
[Abstract]  Small intestinal organoids, otherwise known as enteroids, have become an increasingly utilized model for intestinal biology in vitro as they recapitulate the various epithelial cells within the intestinal crypt (Mahe et al., 2013; Sato et al., 2009). Assessment of growth dynamics within these cultures is an important step to understanding how alterations in gene expression, treatment with protective and toxic agents, and genetic mutations alter properties essential for crypt growth and survival as well as the stem cell properties of the individual cells within the ... [摘要]  小肠类器官,也称为肠袢,已经成为越来越多地用于肠道生物学的体外模型,因为它们重现了在肠隐窝内的各种上皮细胞(Mahe等人 ,2013; Sato et al。,2009)。在这些培养物中评估生长动力学是理解基因表达的改变,用保护性和毒性试剂处理以及遗传突变改变隐窝生长和存活所必需的性质以及细胞内单个细胞的干细胞性质的重要步骤地穴。该方案描述了在隐窝内三维的增殖细胞的可视化方法(Barrett等人,2015)。使用EdU掺入的肠衣的整体增殖染色使得研究者能够观察到肠内的所有增殖细胞,而不是如在包埋和切片中所见到的在薄切片中获得生长信息,确保来自干细胞区室的增殖的真实表现到隐窝的终末分化细胞。

Extraction of Apoplastic Wash Fluids and Leaf Petiole Exudates from Leaves of Arabidopsis thaliana
Author:
Date:
2015-12-20
[Abstract]  The long-distance translocation of metabolites and mineral elements is crucial for plant growth and reproduction. In most cases, source-to-sink translocation of metabolites and minerals requires their passage through the apoplast, irrespective whether they are transported via the xylem or the phloem. This apoplast-mediated pathway is of particular importance during plant senescence, when photoassimilates as well as organic, inorganic or chelated forms of nutrients are translocated from leaves to fruits or seeds. Recent genetic and physiological studies revealed the involvement of numerous ... [摘要]  代谢物和矿物元素的长距离移位对于植物生长和繁殖是至关重要的。在大多数情况下,代谢物和矿物质的源到汇转移需要它们通过质外体,不管它们是通过木质部或韧皮部转运。这种质外体介导的途径在植物衰老期间特别重要,当光同化物以及有机,无机或螯合形式的营养物从叶子转移到水果或种子时。最近的遗传和生理研究揭示了许多膜转运蛋白介导氨基酸,糖,尿素或矿物元素的韧皮部载荷的参与。为了评估单个转运蛋白对木质部卸载或韧皮部负荷的贡献,外质体液和韧皮部液体的收集是必需的。在这里,我们描述了从拟南芥叶中提取质外体液和叶柄渗出物的方法,后者代表韧皮部汁液的真实组成的近似。

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