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Fetal Bovine Serum – TET Tested

胎牛血清

公司名称: Atlanta Biologicals
产品编号: S10350
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Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’)
Author:
Date:
2012-02-05
[Abstract]  Bone marrow derived macrophages are a type of white blood cell that can be isolated from mammalian bone marrow. In this protocol, a method is described in which bone marrow cells are isolated from mouse leg bones (femur and tibia), and then differentiated to bone marrow-derived macrophages in approximately 10-day culture using L929-conditioned medium. [摘要]  通过这种方法来从小鼠的腿骨(股骨和胫骨)中分离骨髓细胞,然后通过L929-条件培养基培养大概十天的时间,从而得到由骨髓细胞分化而成的巨噬细胞。

siRNA Transfection of Mouse Bone Marrow-derived Macrophages
Author:
Date:
2011-02-05
[Abstract]  Short interfering RNAs (siRNA) are a type of double-stranded RNA molecule, typically 20-25 bp long, that are involved in the phenomenon of RNA interference. siRNA transfection is employed in this protocol to knockdown target gene expression in BMM’phi’ cells. Two days after transfection with cells at 60-80% confluence, the knockdown efficiency can reach 90%. [摘要]  siRNA转染是用来阻断BMM细胞中目的基因的表达,转染两天后60-80%细胞聚集, 阻断效率可达到90%。

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