{{'Search' | translate}}
 

Sodium hypochlorite solution

次氯酸钠溶液

公司名称: Sigma-Aldrich
产品编号: 239305
Bio-protocol()
Company-protocol()
Other protocol()

Pathogenicity Assay of Penicillium expansum on Apple Fruits
Author:
Date:
2017-05-05
[Abstract]  Penicillium expansum, a widespread filamentous fungus, is a major causative agent of fruit decay and leads to huge economic losses during postharvest storage and shipping. Furthermore, it produces mycotoxin on the infected fruits that may cause harmful effects to human health. This pathogenicity assay involves a stab inoculation procedure of P. expansum on apple fruit, an important experimental technique to study fungal pathogenesis. This assay can be applied to analyze the virulence of postharvest pathogen on other fruits such as orange, pear and kiwifruit. [摘要]  广泛的青霉菌是广泛的丝状真菌,是果实腐烂的主要致病因素,并在收获后储存和运输中导致巨大的经济损失。此外,它对感染的水果产生霉菌毒素,可能对人体健康造成有害影响。这种致病性测定涉及P的刺刺接种程序。膨化在苹果果实上,是一种重要的实验技术,用于研究真菌发病机制。该测定可用于分析其他水果如橙子,梨和猕猴桃的收获后病原体的毒力。

背景 青霉菌是一种破坏性的采后病原体,在采后处理和储存过程中,许多流行的水果如苹果和梨都会腐烂。造成重大的社会经济影响,对国际贸易产生影响。病原体也可能导致严重的人体健康问题,因为它会产生有毒的次级代谢物,包括patulin,citrinin和球形青霉素(Andersen等,2004)。控制由P引起的腐蚀。膨胀对于确保各种水果的质量和安全性至关重要。
  &nbspConidia的 P。膨胀通常通过创伤进入,这是为提供病原体开发起点所必需的(Spotts等人,1998)。 P的致病性通常通过针刺接种来测试果实上的膨胀,其也用于灰葡萄孢与番茄相比,桃花生的致病性测定, 与芒果,相关的小菜蛾(Colletotrichum gloeosporioides) (Liu等人,2012; Shi等人,2012; ...

Plant Tissue Trypan Blue Staining During Phytopathogen Infection
Author:
Date:
2016-12-20
[Abstract]  In this protocol plant tissue is stained with trypan blue dye allowing the researcher to visualize cell death. Specifically this method avoids the use of the carcinogen compound chloral hydrate, making this classical method of staining safer and faster than ever. The protocol is applied specifically to detect cell death on Arabidopsis leaves during the course of infection with necrotrophic fungus Botrytis cinerea. [摘要]  在该方案中,植物组织用台盼蓝染料染色,使研究人员可视化细胞死亡。具体来说,这种方法避免了使用致癌物水合氯醛,使得这种经典染色方法的染色比以往更加安全和快速。该方案专门用于在坏死性真菌Botrytis cinerea感染过程中检测拟南芥叶片上的细胞死亡。

【背景】 检测死植物组织的最常见的方法之一是台盼蓝染色(Keogh等人,1980)。该重氮染料也用于组织学和药物中,以通过允许细胞死亡的观察来测量组织存活力(Keogh等人,1980; Cooksey,2014)。涉及台盼蓝染色的大多数微观方法需要长时间的清除步骤,使用水合氯醛(CHL),一种目前使用的小型有机化合物,如实验动物中的致癌物,麻醉剂和止痛剂(Keogh等, 1980年; Lu和Greco,2006; Salmon等人,1995)。 CHL未经美国FDA批准或欧盟EMA在任何医疗指征( http://www.accessdata.fda.gov/ )。只有250mg或50mg的合唱水合物足以分别产生成人或儿科镇静剂,其毒性也在新生儿中测定( http://www.drugs.com ...

Isolation of Epithelial Cells from Mouse Gastrointestinal Tract for Western Blot or RNA Analysis
Author:
Date:
2012-11-20
[Abstract]  The gastrointestinal (GI) tract is lined by a single layer of epithelial cells which function in secretion, absorption, and digestion. In addition, most GI tract tumors develop from epithelial cells (carcinomas). This protocol describes isolation of the surface epithelium from the underlying stroma, muscular layer and submucosa in the GI tract. In this protocol, epithelial cell adhesions are weekend by chelating Ca +2 ions followed by mechanical separation of the cells by vortexing. Analysis of protein levels and gene expression patterns in isolated epithelial cells versus whole GI tissue ... [摘要]  该实验方案的中文版正在准备中...

产品评论