{{'Search' | translate}}
 

Sequencing Grade Modified Trypsin

测序级改性胰蛋白酶

公司名称: Promega
产品编号: V5113
Bio-protocol()
Company-protocol()
Other protocol()

In-Solution Digestion Of Purified Yeast Protein For LC-MS
Author:
Date:
2011-04-20
[Abstract]  This method describes the preparation of total yeast protein extract for mass spectrometry analysis. The protein extract is digested by trypsin in a solution with strong denaturants. The digested sample is dried and re-constituted in a mixture compatible with HPLC separation. Samples of isobaric labels should be processed in parallel experiments starting from trypsin digestion. [摘要]  该方法为用于质谱分析的总蛋白提取物做准备。蛋白提取物通过含有强变性剂的溶液中的胰蛋白酶进行消化处理,高压液相色谱分离的相匹配的样品干燥后重新形成复合物。具有等压特征的样品从胰蛋白酶消化开始就要设计进行平行对照试验。

产品评论