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Clark Borosilicate Thin Wall with Filament

Clark带长丝的硼硅酸盐薄壁

公司名称: Harvard Apparatus
产品编号: 30-0066
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Labeling of Precursor Granule Cells in the Cerebellum by ex vivo Electroporation
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2013-06-20
[Abstract]  This protocol will be useful to introduce the genes of interest into the cerebellar granule cells at early stages of development. Since the granule cell precursors are localized in the external granule layer before migration, DNA plasmids can be specifically incorporated into the granule cells by injecting DNA solution into the cerebellar fissures followed by application of electric pulses. This technique can be performed prior to the preparation of either dissociated or organotypic culture, which can be used to study the molecular mechanisms of cell migration, axon elongation and ... [摘要]  该方案可用于在发育的早期阶段将感兴趣的基因引入小脑颗粒细胞。 由于颗粒细胞前体在迁移前位于外部颗粒层中,因此通过将DNA溶液注射到小脑裂隙中,然后施加电脉冲,可将DNA质粒特异性地掺入颗粒细胞中。 该技术可以在制备解离或器官型培养物之前进行,其可以用于研究在发育期间细胞迁移,轴突伸长和突触发生的分子机制。

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