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Ultra Microtome

超薄切片机

公司名称: Leica Biosystems
产品编号: Leica Ultracut UCT
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Colon Tissue Immunoelectron Microscopy
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Date:
2013-10-20
[Abstract]  The method described here is intended to study intracellular localization of proteins in colon cells. This protocol was used to localize IRE1β in the endoplasmic reticulum membrane. We used anti-IRE1β antibody raised in guinea pig for this purpose. We also studied the location of BiP (also known as GRP78), with the antibody raised in rabbit. Both antibodies used with appropriate gold particle-conjugated secondary antibodies gave good results. Primary mouse antibodies are not recommended because secondary anti-mouse antibodies also react with the internal mouse epitopes. [摘要]  本文所述的方法旨在研究结肠细胞中蛋白质的细胞内定位。 该方案用于定位IRE1β在内质网膜中。 我们使用在豚鼠中产生的抗IRE1β抗体用于此目的。 我们还研究了在兔中产生的抗体的BiP(也称为GRP78)的位置。 与合适的金颗粒缀合的二抗一起使用的两种抗体都给出了良好的结果。 不推荐初级小鼠抗体,因为次级抗小鼠抗体也与内部小鼠表位反应。

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