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公司名称: ZEISS
产品编号: CL1500ECO
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Isolation and Primary Cell Culture of Mouse Dorsal Root Ganglion Neurons
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2016-04-05
[Abstract]  We here provide a detailed protocol for the isolation and culture of primary mouse sensory neurons. The cell bodies of sensory afferent pseudounipolar neurons are located in dorsal root ganglia (DRGs) along the vertebral column. Dissected mouse DRGs can be dissociated into single cells by enzymatic digestion to obtain primary cultures of mouse sensory neurons as performed in the studies reported by Khaminets et al. (2015). [摘要]  我们在这里提供了详细的协议,用于隔离和培养的主要小鼠感觉神经元。 感觉传入假性极化神经元的细胞体位于沿着脊柱的背根神经节(DRG)中。 解离的小鼠DRG可以通过酶消化解离成单个细胞,以获得小鼠感觉神经元的原代培养物,如Khaminets等人(2015)报道的研究中所进行的。

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