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Author:
Date:
2016-05-05
[Abstract] The Arabidopsis thaliana seed coat produces large amounts of cell wall polysaccharides, which swell out of the epidermal cells upon hydration of the mature dry seeds. While most mucilage polymers immediately diffuse in the surrounding solution, the remaining fraction tightly adheres to the seed, forming a dense gel-like capsule (Macquet et al., 2007). Recent evidence suggests that the adherence of mucilage is mediated by complex interactions between several cell wall components (Griffiths et al., 2014; Voiniciuc et al., 2015a). Therefore, it is important to ...
[摘要] 拟南芥种子产生大量的细胞壁多糖，其在成熟干种子水合时从表皮细胞中膨胀。 虽然大多数粘液聚合物立即在周围溶液中扩散，但剩余部分紧密粘附于种子，形成致密的凝胶状胶囊（Macquet等人，2007）。 最近的证据表明粘液的粘附由几个细胞壁组分之间的复杂相互作用介导（Griffiths等人，2014; Voiniciuc等人，2015a）。 因此，重要的是评估不同的细胞壁突变体如何影响这种粘液性质。 该协议有助于分析顺序提取的粘液部分中的单糖，并且量化每种成分从种子中的脱离。
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