| In vitro Flow Adhesion Assay for Analyzing Shear-resistant Adhesion of Metastatic Cancer Cells to Endothelial Cells
|
|
Author:
Date:
2016-02-20
[Abstract] Hematogenous metastasis is a primary cause of mortality from metastatic cancer. The shear-resistant adhesion of circulating tumor cells to the vascular endothelial cell surface under blood flow is an essential step in cell extravasation and further tissue invasion. This is similar to a process exploited by leukocytes for adhesion to inflamed blood vessels (leukocyte mimicry). The shear resistant adhesion is mediated by high affinity interactions between endothelial adhesion molecules and their counter receptor ligand expressed on circulating cells. Thus, weak interaction results in a rapid ...
[摘要] 血液转移是转移性癌症死亡的主要原因。循环肿瘤细胞对血流内的血管内皮细胞表面的剪切粘附是细胞外渗和进一步组织侵入的必要步骤。这类似于白细胞用于粘附到发炎血管的方法（白细胞模拟）。剪切抗性粘附由内皮粘附分子和其在循环细胞上表达的其受体配体之间的高亲和力相互作用介导。因此，弱相互作用导致循环细胞从内皮快速分离。尽管癌细胞在血源性转移中的血管粘附的关键作用，我们关于该过程的知识由于难以在体外模拟动态流动条件而受到限制。为了更好地了解癌细胞对内皮的剪切抗性粘附，我们开发了一种用于测量在生理流动条件下循环肿瘤细胞对内皮细胞的剪切抗性粘附的方案，通过适应良好确立的流动粘附测定炎症细胞。该技术可用于评价1）癌细胞的抗剪切粘附能力和2）支持癌细胞粘附所必需的内皮粘附分子（Kang等人，2015）。
|
|
|
| Intestinal Differentiation of Human ESCs
|
|
Author:
Date:
2013-12-20
[Abstract] ES cells (ESCs) are pluripotent and offer a good tool to study early embryonic development. Intestinal cells are derived from the definitive endoderm. In contrast to adult tissue stem cells, embryonic development and differentiation from ES cells have not been as well investigated in the intestine. There are four differentiated cell types of non-proliferative epithelial cells: enterocytes, goblet cells, enteroendocrine cells, and Paneth cells. Intestinal stem cells (ISCs) and progenitor cells reside in crypts, proliferate vigorously, and function as the source of differentiated epithelial ...
[摘要] ES细胞（ESC）是多能性的，并且提供了研究早期胚胎发育的良好工具。肠细胞来源于定形内胚层。与成体组织干细胞相反，胚胎发育和胚胎干细胞的分化在肠道中没有得到很好的研究。存在四种分化的细胞类型的非增殖性上皮细胞：肠细胞，杯状细胞，肠内分泌细胞和Paneth细胞。肠干细胞（ISCs）和祖细胞驻留在隐窝中，有力地增殖，并且作为分化的上皮细胞的来源。在这里，我们描述了一种协议，其中分化的细胞类型的肠来自人类胚胎干细胞。在这个协议中，我们描述一个协议，通过与M15，小鼠中肾细胞系共同培养和用两种化合物治疗有效地将小鼠ES细胞分化为表达Cdx2的肠内胚层。使用的化学化合物是BIO和DAPT。 BIO是Gsk3抑制剂，激活Wnt信号通路，DAPT是抑制Notch信号通路的α-分泌酶抑制剂。 BIO和DAPT处理产生来自人ESC的所有代表性细胞谱系，肠细胞，杯状细胞，肠内分泌细胞和paneth细胞。人ESC的方案主要与用于小鼠ESC的方案非常相似，具有一些修改。
|
|
|
| Intestinal Differentiation of Mouse ESCs
|
|
Author:
Date:
2013-12-20
[Abstract] ES cells (ESCs) are pluripotent and offer a good tool to study early embryonic development. Intestinal cells are derived from the definitive endoderm. In contrast to adult tissue stem cells, embryonic development and differentiation from ES cells have not been as well investigated in the intestine. There are four differentiated cell types of non-proliferative epithelial cells: enterocytes, goblet cells, enteroendocrine cells, and Paneth cells. Intestinal stem cells (ISCs) and progenitor cells reside in crypts, proliferate vigorously, and function as the source of differentiated epithelial ...
[摘要] ES细胞（ESC）是多能性的，并且提供了研究早期胚胎发育的良好工具。肠细胞来源于定形内胚层。与成体组织干细胞相反，胚胎发育和胚胎干细胞的分化在肠道中没有得到很好的研究。存在四种分化的细胞类型的非增殖性上皮细胞：肠细胞，杯状细胞，肠内分泌细胞和Paneth细胞。肠干细胞（ISCs）和祖细胞驻留在隐窝中，有力地增殖，并且作为分化的上皮细胞的来源。在这里，我们描述一种协议，其中分化的细胞类型的肠来自小鼠胚胎干细胞。在这个协议中，我们描述一个协议，通过与M15，小鼠中肾细胞系共同培养和用两种化合物治疗有效地将小鼠ES细胞分化为表达Cdx2的肠内胚层。使用的化学化合物是BIO和DAPT。 BIO是Gsk3抑制剂，激活Wnt信号通路，DAPT是抑制Notch信号通路的α-分泌酶抑制剂。 BIO和DAPT治疗产生所有代表性的细胞谱系，肠细胞，杯状细胞，肠内分泌细胞和paneth细胞，来源于小鼠ESC。
|
|
|