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Micro-Spatula

微量刮铲

公司名称: Thermo Fisher Scientific
产品编号: S50821
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Determination of Intra- and Extracellular Glucose in Mycelium of Fusarium oxysporum
Author:
Date:
2016-07-20
[Abstract]  To study alterations in the metabolism and/or in the transport of glucose during Fusarium oxysporum vegetative growth, we determined intracellular glucose levels in different fungal strains, as well as the amount of glucose remaining in the supernatants after growth in synthetic medium (SM) supplemented with either 0.05 or 2.5% glucose. We used the Glucose (GO) Assay Kit (Sigma-Aldrich) following the instructions of the manufacturer with some modifications. The protocol described here can be applied to other filamentous fungi. [摘要]  为了研究尖镰孢营养生长期间葡萄糖的代谢和/或转运中的改变,我们测定了不同真菌菌株中的细胞内葡萄糖水平以及生长后上清液中剩余的葡萄糖量 在补充有0.05或2.5%葡萄糖的合成培养基(SM)中。 我们使用葡萄糖(GO)测定试剂盒(Sigma-Aldrich)按照制造商的指示进行一些修改。 这里描述的协议可以应用于其他丝状真菌。

Determination of Intracellular ATP Levels in Mycelium of Fusarium oxysporum
Author:
Date:
2016-07-20
[Abstract]  Glycolysis provides metabolites for energy production via oxidative phosphorylation during vegetative growth of Fusarium oxysporum. Therefore, determination of intracellular ATP levels might be of valuable help to analyze regulation of glycolysis/gluconeogenesis pathways. The protocol described here can be applied to other filamentous fungi.
[摘要]  糖酵解在玉蜀黍的营养生长期间通过氧化磷酸化提供能量产生的代谢物。 因此,细胞内ATP水平的测定可能有助于分析糖酵解/糖异生途径的调节。 这里描述的协议可以应用于其他丝状真菌

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