| Imaging Thick Lymph Node Tissue Sections
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Author:
Date:
2016-09-20
[Abstract] Our protocol describes a simple procedure for imaging thick lymph node sections by 2-photon microscopy. Lymph nodes are sectioned using a vibratome (vibrating microtome) to produce slices of tissue that can then be stained with fluorescently labeled antibodies. The thick tissue sections (150-200 μm depth) allow for the detection of cell clustering that is typically under-represented in thin sections (10-20 μm) used for conventional confocal microscopy. Application of 2-photon microscopy facilitates imaging through the thick volume of the vibratome sections. In combination with automated image ...
[摘要] 我们的协议描述了一个简单的程序，通过双光子显微镜成像厚淋巴结切片。 使用vibratome（振动切片机）切割淋巴结以产生组织切片，然后可用荧光标记的抗体染色。 厚组织切片（150-200μm深度）允许检测细胞聚集，其通常在用于常规共聚焦显微镜的薄切片（10-20μm）中表现不足。 双光子显微镜的应用有利于成像通过厚的体积的vibratome部分。 与自动图像处理软件组合，厚的淋巴结横截面图像还有助于在组织的相对大的区域内的细胞事件的定量，从而提供关于感兴趣的细胞事件的空间分布的更清晰的图像（例如 。，T细胞聚类）。 该方法也可以容易地应用于其它组织，例如脾或皮肤。
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| Mouse Subependymal Zone Explants Cultured on Primary Astrocytes
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Author:
Date:
2016-07-20
[Abstract] Neuroblast migration is a major component in the process of neuronal circuit assembly. In the rodent postnatal brain, the subependymal zone (SEZ) is the largest neurogenic niche where new neurons are born throughout life. These cells migrate several millimeters following a specific pathway called the rostral migratory stream (RMS) towards the olfactory bulb. Once they reach their final destination, they differentiate mainly as interneurons and integrate into already established neuronal circuits. Along the RMS, neuroblasts use a particular mode of migration known as chain migration. They stay ...
[摘要] 神经母细胞迁移是神经元电路组装过程中的主要组成部分。 在啮齿动物出生后的大脑中，子宫颈区（SEZ）是新生神经元生命中最大的神经源性生态位。 这些细胞沿着称为传播流（RMS）的特定途径朝向嗅球迁移几毫米。 一旦达到其最终目的地，它们主要区分为中间神经元并整合到已建立的神经元回路中。 沿着RMS，神经母细胞使用称为链转移的特定迁移模式。 它们在迁移时保持相互依赖，并与相邻的星形细胞过程密切相关。 为了解剖神经母细胞迁移中涉及的分子和细胞信号，我们开发了一种体外系统，其由在星形胶质细胞单层顶部共培养的经SEZ衍生的外植体组成。
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