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Formaldehyde

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公司名称: Thermo Fisher Scientific
产品编号: F75F-1GAL
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ChIP Assay for Cell Culture
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2011-01-20
[Abstract]  Chromatin immunoprecipitation (ChIP) is a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. Following crosslinking, cells are lysed and the DNA is broken into pieces 0.2-1.0 kb in length by sonication. At this point immunoprecipitation is performed resulting in the purification of protein–DNA complexes. The identity and quantity of the isolated DNA fragments can then be determined by PCR. This protocol describes how to perform a ChIP experiment and can be applied to different types of cell culture. [摘要]  染色质免疫共沉淀反应是通过确定DNA与目的蛋白在基因组中的结合位置,化学交联反应后,进行超声波处理,使细胞裂解以及DNA断裂成0.2–1.0 kb的片段,通过免疫共沉淀反应使蛋白质-DNA复合物纯化,可以通过PCR的方法对DNA片段的鉴定和量化分析。

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