Citrate Synthase from porcine heart

来自猪心脏的柠檬酸合酶

公司名称: Sigma-Aldrich

产品编号: C3260

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Aggregation Prevention Assay for Chaperone Activity of Proteins Using Spectroflurometry

Author:

Manish Bhuwan, Madhuri Suragani, Nasreen Z. Ehtesham and Seyed E. Hasnain,

Date:

2017-01-20

[Abstract] The ability to stabilize other proteins against thermal aggregation is one of the major characteristics of chaperone proteins. Molecular chaperones bind to nonnative conformations of proteins. Folding of the substrate is triggered by a dynamic association and dissociation cycles which keep the substrate protein on track of the folding pathway (Figure 1). Usually molecular chaperones exhibit differential affinities with different conformations of the substrate. With the exception of the sHsp family of molecular chaperones, the shift from a high-affinity binding state to the low-affinity ... [摘要] 稳定其他蛋白质抵抗热聚集的能力是伴侣蛋白质的主要特征之一。分子伴侣与蛋白质的非构象构象结合。通过动态关联和解离循环来触发底物的折叠，这些循环使底物蛋白保持在折叠通路上（图1）。通常分子伴侣表现出与底物不同构象的差异亲和力。除了分子伴侣的sHsp家族之外，从高亲和力结合状态向低亲和力释放状态的转变由ATP结合和水解引发（Haselback和Buchner，2015）。聚集预防测定是一种简单但尚未确定的测定法，用于确定热不稳定蛋白质如麦芽糖糊精葡糖苷酶（MalZ），柠檬酸盐合酶（CS）和Nde I的伴侣活性。这是基于具有伴侣相似活性的蛋白质预防来自热聚集的蛋白质底物（MalZ，CS和Nde I）的前提。在这里，我们描述了使用我们实验室鉴定的两种不同伴侣蛋白，抵抗素和MoxR1的聚合预防测定的详细方案。分析抗性蛋白（hRes）和MoxR1 a结核分枝杆菌蛋白质对预防MalZ /柠檬酸合酶（CS）/ Nde I聚集的影响。

背景
图1.分子伴侣的作用机制。柠檬酸合酶通过越来越多的结构化中间体（I <1>，2 ...

Acetyl-coenzyme A Synthetase (Acs) Assay

Author:

Sara Castaño-Cerezo, Vicente Bernal and Manuel Cánovas,

Date:

2012-09-05

[Abstract] Acetyl-coenzyme A synthethase (Acs, E.C.6.2.1.1) is an acetate activating enzyme widely represented in nature from bacteria to human. Its function is important for cellular catabolism, especially in order to support microbial growth at low concentrations of acetate (<10 mm)="" (castano="" cerezo="">et al., 2011; Castano Cerezo et al., 2009；Renilla et al., 2012). In this protocol, a continuous coupled enzymatic assay for Acs activity is described. Product formation is followed spectrophotometrically by the formation of NADH. The protocol is tailored for E. coli ... [摘要] 乙酰辅酶A合成酶（Acs，E.C.6.2.1.1）是从细菌到人广泛代表的乙酸活化酶。其功能对于细胞分解代谢是重要的，特别是为了支持在低浓度的乙酸盐（<10mm）下的微生物生长（castano cerezo等人，2011;="" castano="" cerezo等人，="" ，2009;="">
首先由Brown等人描述了乙酰辅酶A合成酶（Acs）测定法。（1977）。使用与苹果酸脱氢酶（Mdh）和柠檬酸合酶（Cs）偶联的酶促方法测量Acs活性：乙酸+ AcASH + ATP→乙酰-CoA + AMP（Cs）乙酰 - CoA +草酰乙酸盐→柠檬酸盐+ Co Wash
（Mdh）L-苹果酸盐+ NAD +→草酰乙酸盐+ NADH
净反应：乙酸盐+ ATP + L-苹果酸盐+ NAD +→柠檬酸盐+ AMP + NADH />在测定条件下，Mdh和Cs活性过量，NADH形成速率受Acs活性限制。

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Citrate Synthase from porcine heart

来自猪心脏的柠檬酸合酶

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