| Plasmodium falciparum Rosette Disruption Assay
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Author:
Date:
2013-04-20
[Abstract] Rosetting, i.e. the capacity of Plasmodium falciparum-infected red blood cells (iRBCs) to bind two or more uninfected red blood cells (RBCs) is associated with severe malaria in African children. Disruption of rosettes using small soluble inhibitors or specific antibodies is viewed as an interesting strategy to treat or prevent severe malaria manifestations. The protocol presented here describes an assay to monitor rosette dissociation, validated for the Palo Alto VarO, IT4/R29 and 3D7/PF13 rosetting clones (Vigan-Womas et al., 2011).
[摘要] Rosetting，即恶性疟原虫感染的红细胞（iRBC）结合两种或更多未感染的红细胞（RBC）的能力与非洲儿童中的严重疟疾有关 。 使用小的可溶性抑制剂或特异性抗体破坏玫瑰花结被视为治疗或预防严重疟疾表现的有趣策略。 本文提供的方案描述了监测玫瑰花结解离的测定，验证用于Palo Alto VarO，IT4/R29和3D7/PF13玫瑰花结的克隆（Vigan-Womas等人，2011）。
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| Plasmodium falciparum Rosette Formation Assay
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Author:
Date:
2013-04-20
[Abstract] Rosetting, i.e. the capacity of red blood cells (iRBCs) infected with mature parasite stages to bind two or more uninfected red blood cells (RBCs) is a virulence factor of Plasmodium falciparum. This protocol describes an in vitro assay to monitor rosette formation by P. falciparum-infected red blood cells, including procedures for rosette enrichment, maintenance of rosetting phenotype and assays for rosetting with RBC labeled using lipophilic fluorescent probes.
[摘要] Rosetting，即感染成熟寄生虫阶段以结合两个或更多个未感染的红细胞（RBC）的红细胞（iRBC）的能力是恶性疟原虫的毒力因子。 。 该方案描述了用于通过EM监测玫瑰花结形成的体外试验。 包括玫瑰花结富集程序，玫瑰花结表型的维持和用亲脂性荧光探针标记的RBC形成玫瑰花结的测定法。
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