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Eppendorf 5331 MasterCycler Gradient Thermal Cycler

Eppendorf 5331 MasterCycler梯度热循环仪

公司名称: Marshall Scientific
产品编号: 5331000.010
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Detection and Cloning of Spliced Transcripts by RT-PCR
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Date:
2013-04-20
[Abstract]  Using a Reverse Transcriptase-PCR approach spliced transcripts can be converted to cDNA, amplified and cloned into an expression plasmid. Sequencing of the obtained cDNA allows identification of the splicing events that generated the detected RNA (Grewe et al., 2012).
[摘要]  使用逆转录酶-PCR方法,可以将剪接的转录物转化为cDNA,扩增并克隆到表达质粒中。 获得的cDNA的测序允许鉴定产生检测的RNA的剪接事件(Grewe等,2012)。

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