| Macrophage Polarization by Tumor-induced MDSCs Assay
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Author:
Date:
2016-08-20
[Abstract] Myeloid derived suppressor cells (MDSCs) are a subset of granulocytes (immature myeloid cells) that exploit a variety of mechanism to modulate the innate and adaptive immune system. MDSCs are present normally in the body, but their numbers increase during inflammation and in cancer, promoting an immunosuppressive microenvironment. In addition to MDSCs, macrophages also play an important role during cancer development. There are two subsets of tumor associated macrophages (TAMs): M1 and M2. M1 are “anti-tumor” macrophages that are activated by interferon gamma (IFN-γ) and/or Lipopolysaccharide ...
[摘要] 骨髓衍生的抑制细胞（MDSC）是粒细胞（未成熟骨髓细胞）的子集，其利用多种机制调节先天和适应性免疫系统。 MDSC通常存在于体内，但它们的数量在炎症和癌症期间增加，从而促进免疫抑制微环境。除了MDSC，巨噬细胞也在癌症发展中发挥重要作用。肿瘤相关巨噬细胞（TAM）有两个亚类：M1和M2。 M1是由干扰素γ（IFN-γ）和/或脂多糖（LPS）活化并分泌大量白细胞介素12（IL-12）从而诱导Th1抗肿瘤免疫应答的"抗肿瘤"巨噬细胞。 M2或"致肿瘤发生"巨噬细胞被白介素4（IL-4）和白细胞介素10（IL-10）激活并分泌大量的IL-10，这促进肿瘤进展（Gabrilovich等人， 。，2012）。在肿瘤微环境中MDSC和巨噬细胞之间的相互作用显示增强这些亚群介导的免疫抑制。 MDSC通过产生IL-10而影响TAM，其继而诱导IL-12的下调并将M1极化为M2巨噬细胞。在我们的研究中，我们使用以下方案来评价肿瘤诱导的MDSC将LPS活化的M1极化为M2巨噬细胞的能力（Vences-Catalan等人，2015）。该方案改编自先前的研究（Sinha等人，2007）。
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| Thioglycollate Induced Peritonitis
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Author:
Date:
2011-06-20
[Abstract] Intraperitoneal (i.p.) injection of thioglycollate elicits a robust influx of neutrophils into peritoneal cavity. The trafficking of the cells is believed to be mediated by chemokines CXCL1, CXCL2, and CXCL8 (Call et al., 2001; Cacalano et al., 1994). Thus this model can be used to test the ability of neutrophils to migrate towards these chemokines in bioengineered mouse strains (e.g. knockout or transgenic mice) or the ability of certain molecules to inhibit the chemoattractive activities of these chemokines (e.g. small molecules or inhibitory antibodies). ...
[摘要] 腹膜内注射巯基乙酸盐可以稳定诱导大量的中性白细胞到腹腔中。对这些细胞的运输被认为是通过CXCL1 ，CXCL2, 和 CXCL8等炎症趋化因子 介导的(1, 2)。 因此这一模型可以用于在生物工程小鼠（例如敲除或转基因小鼠)中检测中性白细胞向这些炎症趋化因子移动，或者能够用于检测确定的分子(例如一些小分子或者抑制类抗体)抑制这些因子的化学活性. 这一实验方法被作者成功的用于鉴定病毒的多重的趋化因子抑制剂的功能。
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