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Author:
Date:
2011-06-20
[Abstract] Intraperitoneal (i.p.) injection of thioglycollate elicits a robust influx of neutrophils into peritoneal cavity. The trafficking of the cells is believed to be mediated by chemokines CXCL1, CXCL2, and CXCL8 (Call et al., 2001; Cacalano et al., 1994). Thus this model can be used to test the ability of neutrophils to migrate towards these chemokines in bioengineered mouse strains (e.g. knockout or transgenic mice) or the ability of certain molecules to inhibit the chemoattractive activities of these chemokines (e.g. small molecules or inhibitory antibodies). ...
[摘要] 腹膜内注射巯基乙酸盐可以稳定诱导大量的中性白细胞到腹腔中。对这些细胞的运输被认为是通过CXCL1 ，CXCL2, 和 CXCL8等炎症趋化因子 介导的(1, 2)。 因此这一模型可以用于在生物工程小鼠（例如敲除或转基因小鼠)中检测中性白细胞向这些炎症趋化因子移动，或者能够用于检测确定的分子(例如一些小分子或者抑制类抗体)抑制这些因子的化学活性. 这一实验方法被作者成功的用于鉴定病毒的多重的趋化因子抑制剂的功能。
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