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4-Methylumbelliferone

4-甲基伞形酮

公司名称: Sigma-Aldrich
产品编号: M1381
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Chitinase Assay from Cultured Bone Marrow Derived Macrophages
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2013-12-05
[Abstract]  Chitinases are chitin-degrading enzymes. Chitinases play essential roles in combating chitin-containing pathogens as well as established roles in asthmatic inflammation. This assay is designed to detect chitinase activity in macrophage cell lysates. The chitin substrate is labeled with 4-methylumbelliferone. Hydrolysis of chitin releases 4-methylumbelliferone, and is measured fluorometrically to determine chitinase activity. [摘要]  几丁质酶是几丁质降解酶。 几丁质酶在对抗含几丁质的病原体以及在哮喘性炎症中确立的作用中起重要作用。 该测定设计用于检测巨噬细胞裂解物中的几丁质酶活性。 几丁质底物用4-甲基伞形酮标记。 几丁质的水解释放4-甲基伞形酮,并通过荧光测定法测定几丁质酶活性。

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