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Author:
Date:
2014-08-20
[Abstract] Measuring antigen-specific T cell responses in the blood and lymphoid organs of vaccinated mice can give us a useful indication of the potency of a vaccine formulation. Unfortunately, systemic or even localized lymphoid T cell responses are not always predictive of the ability of a vaccine to induce tumor protection. Measuring the antigen-specific T cell response within the tumor infiltrating lymphocytes is a more accurate indicator or vaccine efficacy. However, multi-parameter flow cytometric analysis of T cells isolated from tumor tissue can be quite challenging due to the over-whelming ...
[摘要] 测量接种疫苗的小鼠的血液和淋巴器官中的抗原特异性T细胞应答可以给予我们疫苗制剂的效力的有用指示。不幸的是，全身性或甚至局部的淋巴T细胞应答并不总是预示疫苗诱导肿瘤保护的能力。测量肿瘤浸润淋巴细胞内的抗原特异性T细胞应答是更准确的指示或疫苗功效。然而，从肿瘤组织分离的T细胞的多参数流式细胞术分析可能是相当具有挑战性的，因为相对于肿瘤浸润性淋巴细胞（TIL）存在的肿瘤细胞的数量过多，以及与大的和粘附性质相关的问题的许多肿瘤细胞。在这里我们利用使用MACS磁性细胞分选系统从肿瘤组织中预流动分离CD45 +白细胞，产生更清洁的细胞制备物，进行流式细胞染色和分析。
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