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VEGETABLE JUICE

VEGETABLE JUICE

公司名称: Campbell Soup Company
产品编号: V8®ORIGINAL
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Fusarium graminearum Maize Stalk Infection Assay and Associated Microscopic Observation Protocol
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2016-12-05
[Abstract]  The ascomycete fungus Fusarium graminearum (previously also called Gibberella zeae) causes Gibberella stalk rot in maize (Zea mays) and results in lodging and serious yield reduction. To develop methods to assess the fungal growth and symptom development in maize stalks, we present here a protocol of maize stalk inoculation with conidiospores of fluorescent protein-tagged F. graminearumand microscopic observation of the stalk infection process. The inoculation protocol provides repeatable results in stalk rot symptom development, and allows ... [摘要]  子囊菌真菌禾本科禾谷镰菌(以前也称为玉蜀黍赤霉)导致玉米( Zea mays )中的赤霉菌并导致倒伏和严重的产量降低。为了开发方法来评估玉米茎的真菌生长和症状发展,我们在这里提出玉米茎接种与荧光蛋白标记的F的分生孢子的协议。禾谷镰刀菌和茎显影观察茎感染过程。该接种方案在茎腐病症发展中提供可重复的结果,并且允许跟踪在细胞规模的玉米茎中的真菌菌丝生长。
关键字: 镰孢镰孢茎侵蚀,植物真菌病原体,接种。

[背景] 玉米( Zea mays )是整个土壤中最重要的作物之一世界。茎是玉米植物的主茎。它由节点和节点组成(图1)。节间包括皮和髓,并且血管组织分散在髓中。图1显示了在明场或GFP荧光通道下的玉米茎部分的显微图像。这为玉米秆接种和观察提供了背景。  子囊菌真菌禾本科镰刀菌(以前也称为玉蜀黍赤霉)会导致玉米中的赤霉菌腐烂( Zea mays >),并导致倒伏和产量损失(Jackson等人,2009; Santiago等人,2007)。 ...

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