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Flow cell, rectangular, UV quartz, 6.5 x 11 mm aperture, 1 mm pathlength, M6 thread, 400 mm inlet/outlet tubes, 113 μL, for Cary 50/60 only

流动池,矩形,UV石英,6.5×11mm孔径,1mm路径长度,M6螺纹,400mm入口/出口管,113μL,仅用于Cary 50/60

公司名称: Agilent Technologies
产品编号: 6610019900
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Isolation of Polysome-bound mRNA from Rice Solid Tissues Amenable for RT-PCR and Profiling Experiments
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2015-03-05
[Abstract]  Polysome profile analysis is a frequently performed task in translational control research that not only enables direct monitoring of the efficiency of translation but can easily be extended with a wide range of downstream applications such as Northern and western blotting, genome-wide microarray analysis or qRT-PCR. Here, we describe a method for the isolation and quantification of high-quality polysome-bound mRNA complexes from small quantities of liquid-nitrogen-frozen solid tissue samples of rice shoots/roots. The mRNA obtained can be further analyzed by methods that evaluate polysomal ... [摘要]  Polysome谱分析是翻译控制研究中经常执行的任务,不仅能够直接监测翻译的效率,而且可以很容易地扩展到广泛的下游应用,如Northern和western印迹,全基因组微阵列分析或qRT- PCR。 在这里,我们描述了一种从少量液氮冷冻固体组织样本的水稻芽/根中分离和定量高质量多糖结合mRNA复合物的方法。 获得的mRNA可以通过在单个转录物或全局水平评估多核糖体mRNA丰度的方法进一步分析。

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