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Tryptone

胰蛋白胨

公司名称: Teknova
产品编号: T9012
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Airbrush Infiltration Method for Pseudomonas syringae Infection Assays in Soybean
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Date:
2015-03-20
[Abstract]  We developed this protocol to assay the extent of proliferation of Pseudomonas syringae pv. glycinea in soybean leaves. This method specifically enables accurate pathogenesis assays of soybean plants at V2/V3 (2nd/3rd trifoliate) or higher stages of growth. The leaves of soybean plants at these growth stages are not amenable to bacterial infiltration using routine needleless syringe infiltration due to the high number of trichomes on these mature leaves. This method enables efficient infiltration of bacteria into the epidermal cells of mature leaves ... [摘要]  我们开发了该方案来测定丁香假单胞菌pv的增殖程度。 在大豆叶中。 该方法特异性地能够在V2/V3(2期/3期/3期/3期/3期/3期)或更高生长阶段进行大豆植物的准确发病机理测定。 由于这些成熟叶上的大量毛状体,大豆植物在这些生长阶段的叶子不适于使用常规无针注射器浸润的细菌浸润。 该方法使用压力泵能够有效地将细菌浸润到成熟叶的表皮细胞中。

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