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NuncTM Cell-Culture Treated Multidishes

Nunc TM细胞培养处理的多层

公司名称: Thermo Fisher Scientific
产品编号: 140685
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13C Kinetic Labeling and Extraction of Metabolites from Adherent Mammalian Cells
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2015-04-20
[Abstract]  Fluctuations in metabolite levels in mammalian cells are the most direct form of readout of the cellular metabolic state. The current protocol describes a method for pulse labeling and subsequent isolation of metabolites from adherent mammalian cells. The isolated metabolites can be identified and quantified by mass-spectrometry, allowing for estimation of the rates of synthesis and removal of metabolites from the system being analyzed. [摘要]  哺乳动物细胞中代谢物水平的波动是细胞代谢状态的最直接形式的读出。 当前的协议描述了用于脉冲标记和随后从贴壁哺乳动物细胞分离代谢物的方法。 分离的代谢物可以通过质谱法鉴定和定量,允许估计代谢物从所分析的系统的合成速率和去除速率。

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