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Author:
Date:
2016-11-05
[Abstract] Allogeneic organ transplantation is a powerful tool for clinical and basic research studies. However, the graft is often rejected by the host organism. Here, we describe a protocol that uses immunodeficient rag1 mutant zebrafish. These zebrafish escaped rejection, which made it possible to successfully transplant fragments of an allogeneic testis and testicular hyperplasia. This protocol can be used to amplify and maintain testicular hyperplasia grafts for several years (Kawasaki et al., 2016). The amplified hyperplasias are likely to be a good source of somatic and germ ...
[摘要] 细胞色素（Cyt）b 559 是光合电子传递系统中光系统II的重要和必需的核心组分，是一种血红素桥接异二聚体蛋白，由α亚基 PsbE ）和β亚基（ PsbE ），其还原形式在559nm的α波段具有吸收最大值。通过用等吸光度点在580nm处的吸光度归一化的减少氧化的差异光谱的分光光度测量可以测定Cyt b 559的量。作者使用的差别消光系数为Cyt b 559 [Δε（559-580nm）= 15.5mM -1 > cm -1 ]，这已由Garewal和Wasserman（1974）报道。除了Cyt b559之外，该程序可用于Cyt b 和Cyt 的定量，Cyt > b 6 / f 复合体和P 700 改编自Fujita和Murakami（1987），用于蓝细菌，细长聚球藻PCC 7942，以及其他蓝细菌菌株，包括集胞藻属（Synechocystis）。 PCC 6803。...
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