Heavy Metal Stress Assay of Caenorhabditis elegans
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Author:
Date:
2017-06-05
[Abstract] Organisms have developed many protective systems to reduce the toxicity from heavy metals. The nematode Caenorhabditis elegans has been widely used to determine the protective mechanisms against heavy metals. Responses against heavy metals can be monitored by expression of reporter genes, while sensitivity can be determined by quantifying growth or survival rate following exposure to heavy metals.
[摘要] 生物开发了许多保护系统,以减少重金属的毒性。线虫秀丽隐杆线虫已广泛用于确定重金属的保护机制。可以通过报告基因的表达来监测对重金属的反应,而敏感性可以通过量化暴露于重金属后的生长或存活率来确定。
背景 一些重金属如砷,镉和汞已知对包括人类在内的大多数生物有害(Valko et al。,2005)。为了降低这些金属的毒性,生物开发出各种保护系统。线虫秀丽隐杆线虫已被用于了解重金属的保护机制。以前的研究表明,许多基因,如解毒酶,转录因子和信号传导因子都参与了该生物体中重金属的保护(Broeks et al。,1996; Mizuno et al。 ,2004; Inoue等人,2005; Schwartz等人,2010)。除了测量报告基因表达外,生存力和生长的测定通常用于监测重金属在C中的影响。线虫。在本协议中,我们描述了使用C测定砷,铜和镉的方法。线虫。
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Extraction and Measurement of Abscisic Acid in a Unicellular Red Alga Cyanidioschyzon merolae
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Author:
Date:
2016-12-05
[Abstract] Abscisic acid (ABA) has been known as a phytohormone of land plants, which is synthesized in response to abiotic stresses and induces various physiological responses, but is also found from eukaryotic algae. Recently, we reported that a unicellular red alga Cyanidioschyzon merolae produced ABA, which prevented cell growth and enhanced salt stress tolerance (Kobayashi et al., 2016). This report describes the detailed method for the extraction and quantification of ABA in the model red alga C. merolae.
[摘要] 脱落酸(ABA)已知为陆生植物的植物激素,其响应于非生物胁迫合成并诱导各种生理反应,但也可从真核藻类中发现。最近,我们报道了单细胞红藻(Cyanidioschyzon merolae)产生ABA,其阻止细胞生长和增强盐胁迫耐受性(Kobayashi等人,2016)。该报告描述了在红海藻模型中提取和定量ABA的详细方法。 。 关键字:脱落酸,藻类, Cyanidioschyzon merolae ,HPLC,植物激素 ] 植物激素ABA已在发散光合真核生物中发现,但单细胞藻类的功能仍不清楚。在最近的研究中,我们显示单细胞红藻。 melorae 通过本方案累积ABA以应答盐胁迫。这是用于从C中提取和定量ABA的第一已公布方案的细节。 merolae 。此协议针对 C进行了优化。基于陆地植物协议的。
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