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Ampicillin, trihydrate

公司名称: Sangon Biotech
产品编号: A100741
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Expression and Purification of the GRAS Domain of Os-SCL7 from Rice for Structural Studies
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2017-02-05
[Abstract]  GRAS proteins, named after the first three members GAI, RGA and SRC, has been found in 294 embryophyta species and is represented by 1,035 sequences. They belong to a plant-specific protein family and play essential roles in plant growth and development. Proteins in this family are defined as minimally containing a conserved GRAS domain, which is about 350-450 resides and can be subdivided into five distinct motifs with their name derived from the most prominent amino acids: LRI (leucine-rich region I), VHIID, LRII (leucine-rich region II), PFYRE and SAW and mainly function in the interaction ... [摘要]  以前3个成员GAI,RGA和SRC命名的GRAS蛋白质已被发现在294个胚胎种,并由1,035个序列表示。它们属于植物特异性蛋白质家族,在植物生长和发育中起重要作用。该家族中的蛋白质被定义为最低限度地含有保守的GRAS结构域,其约为350-450个,并且可以细分为五个不同的基序,其名称源自最突出的氨基酸:LRI(富含亮氨酸的区域I),VHIID ,LRII(富含亮氨酸的区域II),PFYRE和SAW,并且主要在GRAS蛋白质与其配偶体之间的相互作用中起作用(Sun等人,2012)。通过系统发育分析,GRAS家族可以分为十多个亚科,其中SCL4 / 7是一个重要的亚组,对应对环境压力有作用。这里我们描述了Os-SCL7(水稻SCL4 / 7成员)的GRAS结构域的表达和纯化的详细方案,使我们能够使其结晶并确定其结构。

背景 GRAS蛋白是一个大家族,在植物发育和信号转导中起重要作用。结果表明,一些家庭成员如DELLAs起到GA响应植物生长的抑制作用,是GA信号通路(Murase等人,2008)中的关键调控目标,NSP1和NSP2起重要作用在调节结瘤发育和信号传导(Kaló等人,2005)中,蛋白质SCR和SHR一起在控制根和芽的径向图案中起重要作用(Helariutta et ...

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