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OC-725C

公司名称: Warner Instruments
产品编号: Oocyte Clamp OC-725C
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Two-electrode Voltage-clamp Recordings in Xenopus laevis Oocytes:Reconstitution of Abscisic Acid Activation of SLAC1 Anion Channel via PYL9 ABA Receptor
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2017-01-20
[Abstract]  Two-Electrode Voltage-Clamp (TEVC) recording in Xenopus laevis oocytes provides a powerful method to investigate the functions and regulation of ion channel proteins. This approach provides a well-known tool to characterize ion channels or transporters expressed in Xenopus laevis oocytes. The plasma membrane of the oocyte is impaled by two microelectrodes, one for voltage sensing and the other one for current injection. Here we list a protocol that allows robust reconstitution of multi-component signaling pathways. This protocol has been used to study plant ion channels, ... [摘要]  在非洲爪蟾卵母细胞中记录的双电极电压钳(TEVC)提供了一种强大的方法来研究离子通道蛋白的功能和调节。这种方法提供了一种众所周知的用于表征在非洲爪蟾卵母细胞中表达的离子通道或转运蛋白的工具。卵母细胞的质膜由两个微电极引起,一个用于电压感测,另一个用于电流注入。在这里,我们列出了一个允许多组分信号通路强制重组的协议。该方案已经用于研究植物离子通道,包括SLAC1通道(SLOW ANION CHANNEL-ASSOCIATED 1),特别是SLAC1通过蛋白激酶OST1(OPEN STOMATA 1),Ca 2 +依赖性蛋白激酶(CPK)或GHR1(GUARD细胞过氧化氢抗性1)跨膜受体样蛋白。显示了通过“单体”ABA(脱落酸)受体RCAR1 / PYL9(PYRABACT INRESISTANCE1 [PYR1] / PYR1-样[PYL] / ABA受体[RCAR]的调节因子]重建SLAC1阴离子通道的脱落酸活化的数据。通过共表达脱落酸信号核心的四个组分。该方案也适用于研究其他离子通道功能和调节机制,以及转运蛋白。背景 ...

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