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SeraMir Exosome RNA Purification Column kit (20 exoRNA columns)

公司名称: System Biosciences
产品编号: RA808A-1
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miRNA Characterization from the Extracellular Vesicles
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2017-02-20
[Abstract]  Cancer cells and cancer associated stromal cells co-evolve secrete extracellular vesicles to the surrounding regions and regulate several processes involved in cancer metastasis. miRNAs have been known to be mediators of cancer progression and metastasis. miRNAs consist of short noncoding RNA. miRNAs are stable in extracellular fluids such as serum, plasma and urine. miRNAs are secreted by cells in normal and diseased conditions. miRNAs signatures have been identified specific to certain disease conditions. Therefore they are valuable biomarkers for different diseases. In our study we ... [摘要]  癌细胞和癌症相关的基质细胞将分泌的细胞外囊泡共同进入周围区域并调节参与癌症转移的几个过程。已知miRNA是癌症进展和转移的介质。 miRNA由短的非编码RNA组成。 miRNA在细胞外液体如血清,血浆和尿液中是稳定的。 miRNA在正常和患病条件下由细胞分泌。已经鉴定了特定疾病条件的miRNAs特征。因此,它们是不同疾病的宝贵生物标志物。在我们的研究中,我们确定了某些miRNAs,miR-409-3p和miR-409-5p,其由激活的基质成纤维细胞分泌并被癌细胞摄取以诱导爆发性肿瘤生长,通过激活癌细胞的上皮至间质转化细胞。在这里,我们描述了一种在细胞外囊泡中测定miRNA(miR-409-3p和miR-409-5p)的方法,其由表达miR-409的前列腺癌基质细胞分泌。在该方法中,使用来自间质成纤维细胞的条件培养基来提取囊泡部分。从囊泡部分纯化RNA,并使用实时PCR测定将特异性miRNA逆转录并定量。

背景 miRNA是大小为20-23个核苷酸的短非编码RNA。已经在组织和体液中检测到miRNA。已经使用Northern印迹和定量实时PCR测定了miRNA表达水平。 ...

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