Chondroitin sulfate sodium salt from shark cartilage

公司名称: Sigma-Aldrich

产品编号: C4384

A New, Rapid Method for the Quantification of Dolichyl Phosphates in Cell Cultures Using TMSD Methylation Combined with LC–MS Analysis

Dipali Kale, Timo Sachsenheimer, Albert Sickmann and Britta Brügger

Published online: 2023-11-20

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Heparan Sulfate Identification and Characterisation: Method I. Heparan Sulfate Identification by NMR Analysis

Author:

Susan M. Carnachan and Simon F.R. Hinkley,

Date:

2017-04-05

[Abstract] Heparin and heparan sulfate (HS) may be purified from complex biological matrices and are often isolated in sub-milligram quantities but not unequivocally identified by spectroscopic means. This protocol details a methodology to rapidly assess the gross compositional features and approximate purity of HS by ¹H nuclear magnetic resonance. A complimentary method for identification and characterisation of heparan sulfate can be found at Carnachan and Hinkley (2017). [摘要] 肝素和硫酸乙酰肝素（HS）可以从复杂的生物学基质中纯化出来，通常以亚毫克数量分离，但不能通过光谱学方法明确鉴定。该方案详细介绍了一种方法，通过H核磁共振快速评估HS的总体组成特征和近似纯度。 Carnachan和Hinkley（2017）可以找到一种免费的硫酸乙酰肝素鉴定和表征方法。

存在一些用于肝素和HS分析的方法。该方案旨在提供一种可重现和广泛应用的通过核磁共振（NMR）快速鉴定肝素/ HS的方法。可以以非破坏性的方式容易地评估小样品（〜0.3mg），以确定纯化肝素样分子时生物样品中发现的其他常见污染物的近似纯度。本文描述的方法旨在提供适用于没有经验的糖胺聚糖（GAG）分析的实验室的逐步方案。

Isolation and Analysis of Proteoglycans and Glycosaminoglycans from Archaeological Bones and Teeth

Author:

Yvette May Coulson-Thomas, Vivien Jane Coulson-Thomas and Andrew L. Norton,

Date:

2017-04-05

[Abstract] We have developed methods for isolating proteoglycans and glycosaminoglycans from archaeological bones and teeth. These methods have been previously published (Coulson-Thomas et al., 2015) and are described here in more detail. In the case of glycosaminoglycans, the method was a previously described method (Nader et al., 1999) which we optimized for archeological samples. [摘要] 我们开发了从考古学骨骼和牙齿中分离蛋白聚糖和糖胺聚糖的方法。以前已经公开了这些方法（Coulson-Thomas等人，2015），并且在这里更详细地描述。在糖胺聚糖的情况下，该方法是先前描述的方法（Nader等人，1999），其针对考古样品进行了优化。
【背景】骨组织主要由矿物成分（羟基磷灰石）和由胶原，非胶原蛋白和蛋白聚糖（PG）组成的有机基质组成。由于与羟基磷灰石紧密结合，细胞外基质蛋白和PG被保护免受死亡后温度和化学药剂的破坏作用。然而，到目前为止，只有DNA和蛋白质已经从考古学骨骼中成功提取，因此我们开发了从考古学骨骼和牙齿中分离PG和糖胺聚糖（GAG）链的方法。 PGs和GAG在骨形态发生，体内平衡和退行性骨病中起主要作用，从考古骨骼分析这些分子将揭示有价值的古生物学信息。

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Chondroitin sulfate sodium salt from shark cartilage

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