Dermatan sulfate

公司名称: Sigma-Aldrich

产品编号: 1171455

A New, Rapid Method for the Quantification of Dolichyl Phosphates in Cell Cultures Using TMSD Methylation Combined with LC–MS Analysis

Dipali Kale, Timo Sachsenheimer, Albert Sickmann and Britta Brügger

Published online: 2023-11-20

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Isolation and Analysis of Proteoglycans and Glycosaminoglycans from Archaeological Bones and Teeth

Author:

Yvette May Coulson-Thomas, Vivien Jane Coulson-Thomas and Andrew L. Norton,

Date:

2017-04-05

[Abstract] We have developed methods for isolating proteoglycans and glycosaminoglycans from archaeological bones and teeth. These methods have been previously published (Coulson-Thomas et al., 2015) and are described here in more detail. In the case of glycosaminoglycans, the method was a previously described method (Nader et al., 1999) which we optimized for archeological samples. [摘要] 我们开发了从考古学骨骼和牙齿中分离蛋白聚糖和糖胺聚糖的方法。以前已经公开了这些方法（Coulson-Thomas等人，2015），并且在这里更详细地描述。在糖胺聚糖的情况下，该方法是先前描述的方法（Nader等人，1999），其针对考古样品进行了优化。
【背景】骨组织主要由矿物成分（羟基磷灰石）和由胶原，非胶原蛋白和蛋白聚糖（PG）组成的有机基质组成。由于与羟基磷灰石紧密结合，细胞外基质蛋白和PG被保护免受死亡后温度和化学药剂的破坏作用。然而，到目前为止，只有DNA和蛋白质已经从考古学骨骼中成功提取，因此我们开发了从考古学骨骼和牙齿中分离PG和糖胺聚糖（GAG）链的方法。 PGs和GAG在骨形态发生，体内平衡和退行性骨病中起主要作用，从考古骨骼分析这些分子将揭示有价值的古生物学信息。

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