Sodium sulphite

公司名称: Carl Roth

产品编号: P033

Preparation and Purification of β-1,3-glucan-Linked Candida glabrata Cell Wall Proteases by Ion-Exchange Chromatography, Gel Filtration, and MDPF-Gelatin-Zymography Assay

Pirjo Pärnänen, Timo Sorsa, Taina Tervahartiala and Pirjo Nikula-Ijäs

Published online: 2024-03-20

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Measurement of the Galactanase Activity of the GanB Galactanase Protein from Bacillus subtilis

Author:

Hildegard Watzlawick,

Date:

2017-04-05

[Abstract] The activity of the endo-β-1,4-galactanase GanB from B. subtilis on the high molecular weight β-1,4-galactan was determined quantitatively by the measurement of the increase of the reducing power or with the dyed substrate Azo-galactan. The generated degradation products were analyzed using thinlayer-chromatography (TLC) or high-performance anion-exchange chromatography (HPAEC). [摘要] 来自B的内切-β-1,4-半乳糖苷酶GanB的活性。通过测量还原力的增加或染色底物偶氮半乳聚糖来定量测定高分子量β-1,4-半乳聚糖上的枯草芽孢杆菌。使用薄层色谱（TLC）或高效阴离子交换层析（HPAEC）分析产生的降解产物。

枯草芽孢杆菌具有用于植物细胞壁多糖的综合系统，包括编码半乳聚糖利用元素的基因簇ganSPQAB（Watzlawick等人，2016）。半乳聚糖是高分子量半乳糖，并且在果胶中被认为是I型鼠李糖半乳糖醛酸的侧链。其降解通过内切β1,4半乳聚糖酶（EC 3.2.1.89）进行。半乳聚糖的利用。枯草芽孢杆菌涉及细胞外半乳聚糖酶GanB，裂解链内的高分子半乳聚糖，并产生进入细胞壁的短的低聚物进一步降解。来自的 ganB 基因。枯草芽孢杆菌在大肠杆菌（Watzlawick等人，2016）中克隆并表达，纯化的His标记的成熟蛋白的酶学性质由半乳聚糖酶测定。

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Sodium sulphite

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