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Midi PlusTM Pipetting Controller

公司名称: Sartorius
产品编号: 710931
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Analysis of Mitochondrial Transfer in Direct Co-cultures of Human Monocyte-derived Macrophages (MDM) and Mesenchymal Stem Cells (MSC)
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2017-05-05
[Abstract]  Mesenchymal stem/stromal cells (MSC) are adult stem cells which have been shown to improve survival, enhance bacterial clearance and alleviate inflammation in pre-clinical models of acute respiratory distress syndrome (ARDS) and sepsis. These diseases are characterised by uncontrolled inflammation often underpinned by bacterial infection. The mechanisms of MSC immunomodulatory effects are not fully understood yet. We sought to investigate MSC cell contact-dependent communication with alveolar macrophages (AM), professional phagocytes which play an important role in the lung inflammatory ... [摘要]  间充质干/基质细胞(MSC)是成年干细胞,已被证明可以改善急性呼吸窘迫综合征(ARDS)和败血症临床前模型中的存活,增强细菌清除和减轻炎症。这些疾病的特征在于通常受细菌感染支持的不受控制的炎症。 MSC免疫调节作用的机制尚未完全了解。我们试图研究与肺泡巨噬细胞(AM),在肺炎症反应和抗菌防御中起重要作用的专业吞噬细胞的MSC细胞接触依赖性通信。通过使用基本的直接共培养系统,共聚焦显微镜和流式细胞术,我们通过隧道纳米管(TNT)显现并有效量化了MSC线粒体转移到AM。为了模拟人类AM,在粒细胞巨噬细胞集落刺激因子(GM-CSF)存在下,从人供体血液中分离原代单核细胞并分化成巨噬细胞(单核细胞衍生的巨噬细胞,MDM),从而允许适应AM样表型(de Almeida等人,2000; Guilliams等人,2013)。人骨髓衍生的MSC用线粒体特异性荧光染色标记,广泛洗涤,以1:20(MSC / ...

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