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Thin Wall Glass Capillaries; 4 in. (100 mm); 1 / 0.75 OD/ID (mm); Fire Polished; 500 capillaries

公司名称: World Precision Instruments
产品编号: TW100-4
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Zebrafish Embryo Xenograft and Metastasis Assay
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Date:
2018-09-20
[Abstract]  Xenograft models, and in particular the mouse xenograft model, where human cancer cells are transplanted into immunocompromised mice, have been used extensively in cancer studies. Although these models have contributed enormously to our understanding of cancer biology, the zebrafish xenograft model offers several advantages over the mouse model. Zebrafish embryos can be easily cultured in large quantities, are small and easy to handle, making it possible to use a high number of embryos for each experimental condition. Young embryos lack an efficient immune system. Therefore the injected ... [摘要]  异种移植模型,特别是小鼠异种移植模型,其中人癌细胞被移植到免疫受损小鼠中,已广泛用于癌症研究。尽管这些模型对我们对癌症生物学的理解做出了巨大贡献,但斑马鱼异种移植模型与鼠标模型相比具有多种优势。斑马鱼胚胎可以很容易地大量培养,体积小且易于处理,因此可以在每种实验条件下使用大量胚胎。年轻的胚胎缺乏有效的免疫系统。因此,注射的癌细胞不被排斥,并且原发性肿瘤和微转移的形成是快速的。胚胎的透明度使得能够在完整和活的胚胎中对原发性肿瘤和转移瘤进行成像。在这里,我们描述了一种方法,其中表达GFP的肿瘤细胞被注射到斑马鱼胚胎的心包空间。在注射后4天,对胚胎成像并分析原发性肿瘤和远端微转移的形成。

【背景】 斑马鱼( Danio rerio )是一种小型淡水鱼,不仅在发育生物学中,而且在生物医学中越来越受欢迎。快速发展的领域之一是使用斑马鱼作为癌症生物学研究的模型。斑马鱼高效可靠地交配,可以产生大量小,透明,外部发育的后代,可以在例如,多孔板中轻松培养(White et al。 >,2013)。有趣的是,人类肿瘤细胞可以植入斑马鱼胚胎中,许多异种移植物能够通过在胚胎中生长,侵入和转移来忠实地重演它们的恶性行为(Lee et al。,2005; Nicoli et al。,2007; Chapman et ...

Escherichia coli Infection of Drosophila
Author:
Date:
2017-05-05
[Abstract]  Following septic insults, healthy insects, just like vertebrates, mount a complex immune response to contain and destroy pathogens. The failure to efficiently clear bacterial infections in immuno-compromised fly mutants leads to higher mortality rates which provide a powerful indicator for genes with important roles in innate immunity. The following protocol is designed to reproducibly inject a known amount of non-pathogenic E. coli into otherwise sterile flies and to measure the survival of flies after infection. The protocol can be easily adapted to different types of bacteria. [摘要]  在败血症后,健康的昆虫就像脊椎动物一样,会发生复杂的免疫反应,以遏制和破坏病原体。在免疫损害的蝇突变体中未能有效地清除细菌感染导致更高的死亡率,这为在先天免疫中具有重要作用的基因提供了强有力的指标。以下协议被设计为可重复地注射已知量的非致病性E。大肠杆菌进入其他无菌苍蝇,并测量感染后苍蝇的存活率。该方案可以轻松适应不同类型的细菌。

背景 经典的感染实验包括口服感染果蝇(Chakrabarti等人,2016)或用浸在浓缩细菌溶液中的针(Romeo和Lemaitre,2008)。与这些方案不同,我们的实验程序允许我们确定感染部位,并精确控制注射到每只苍蝇中的细菌的剂量。这提供了均匀性和重复性,并且允许我们适应不同实验的细菌负荷(Akbar等人,2011和2016)。

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