{{'Search' | translate}}
 

Cytarabine

公司名称: Sigma-Aldrich
产品编号: C3350000
Bio-protocol()
Company-protocol()
Other protocol()

Primary Olfactory Ensheathing Cell Culture from Human Olfactory Mucosa Specimen
Author:
Date:
2017-05-20
[Abstract]  The human olfactory mucosa is located in the middle and superior turbinates, and the septum of nasal cavity. Olfactory mucosa plays an important role in detection of odours and it is also the only nervous tissue that is exposed to the external environment. This property leads to easy access to the olfactory mucosa for achieving various researches. The lamina propria of olfactory mucosa consists of olfactory ensheathing cells (OECs) that cover the nerve fibers of olfactory. Here we describe a protocol for isolation of OECs from biopsy of human olfactory mucosa. [摘要]  人类嗅粘膜位于中鼻甲和鼻中隔,鼻腔隔膜。嗅粘膜在气味检测中起重要作用,也是暴露于外界环境的唯一神经组织。该属性可以方便地进入嗅粘膜进行各种研究。嗅粘膜固有层由覆盖嗅神经纤维的嗅鞘细胞(OEC)组成。在这里,我们描述了从人类嗅粘膜活检中分离OEC的方案。

背景 嗅鞘细胞(OECs)是神经胶质细胞,其表达与星形胶质细胞和施旺细胞类似的各种抗原,例如胶质纤维相关蛋白(GFAP),S100-β,p75低亲和力神经生长因子受体,波形蛋白,巢蛋白和神经肽Y (Singh等人,2013)。嗅鞘细胞释放在中枢神经系统的细胞生长和粘连中起作用的不同神经营养因子和粘附分子(Pastrana等人,2007)。此外,这些细胞在受损的中枢神经系统的再生中起重要作用,例如治疗脊髓损伤和神经变性疾病(Novikova等人,2011)。我们选择OEC作为我们研究中的研究材料,因为它们具有诸如高迁移能力,可及源,鼻嗅粘膜干细胞分化和非致瘤性行为等优势性质(Huang et al。 ,2008; Escada 等人,2009)。该方案描述了从人类嗅觉粘膜样品中分离OEC的一步一步的过程。

产品评论