{{'Search' | translate}}
 

ECL Direct Labeling and Detection System

公司名称: Cytiva
产品编号: RPN3000
Bio-protocol()
Company-protocol()
Other protocol()

CRISPR-PCS Protocol for Chromosome Splitting and Splitting Event Detection in Saccharomyces cerevisiae
Author:
Date:
2017-05-20
[Abstract]  Chromosome engineering is an important technology with applications in basic biology and biotechnology. Chromosome splitting technology called PCS (PCR-mediated Chromosome Splitting) has already been developed as a fundamental chromosome engineering technology in the budding yeast. However, the splitting efficiency of PCS technology is not high enough to achieve multiple splitting at a time. This protocol describes a procedure for achieving simultaneous and multiple chromosome splits in the budding yeast Saccharomyces cerevisiae by a new technology called CRISPR-PCS. At least four ... [摘要]  染色体工程是应用于基础生物学和生物技术的重要技术。染色体分裂技术称为PCS(PCR介导的染色体分裂)已经被开发为发芽酵母中的基本染色体工程技术。然而,PCS技术的分割效率不够高,不能一次实现多次分割。该协议描述了通过称为CRISPR-PCS的新技术在芽状酵母酿酒酵母中实现同时和多个染色体分裂的过程。基因组中至少四个独立的位点可以通过一次转化来分裂。与常规PCS技术相比,获得多重分裂酵母菌株的总时间和劳动力大大降低。

背景 能够快速有效地操纵多个遗传基因座或染色体区域的染色体工程技术变得越来越重要。这些技术为阐明染色体和基因组功能提供了有力的手段。此外,它可以用于通过创建广泛的遗传变体来繁殖有用的菌株。在芽殖酵母酿酒酵母(Saccharomyces ...

产品评论