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Vannas Spring Scissors - 2.5Mm Cutting Edge

公司名称: Fine Science Tools
产品编号: 15001-08
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Live Imaging of Myogenesis in Indirect Flight Muscles in Drosophila
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2017-07-05
[Abstract]  The indirect flight muscles (IFMs) are the largest muscles in the fly, making up the bulk of the adult thorax. IFMs in Drosophila are generated during pupariation by fusion of hundreds of muscle precursor cells (myoblasts) with larval muscle templates (myotubes). Prominent features, including the large number of fusion events, the structural similarity to vertebrate muscles, and the amenability to the powerful genetic techniques of the Drosophila system make the IFMs an attractive system to study muscle cell fusion. Here we describe methods for live imaging of IFMs, both in ... [摘要]  间接飞行肌肉(IFM)是飞行中最大的肌肉,构成成年胸部的大部分。 通过将数百种肌肉前体细胞(成肌细胞)与幼虫肌肉模板(肌管)融合,在果蝇中产生IFMs 。 突出的特征,包括大量的融合事件,与脊椎动物肌肉的结构相似性,以及对果蝇系统强大的遗传技术的适应性使得IFM成为研究肌肉细胞融合的有吸引力的系统。 在这里,我们描述了在完整的蛹中和在离体的独立的IFM中实时成像IFM的方法。 (Segal等人,2016年)在手稿中使用了这里阐述的方案。
【背景】胚胎肌肉长期以来一直是肌肉发育研究的建立模型系统(Volk,1999; Chen和Olson,2004; Abmayr等人,2008; Richardson pup biological(Dutta,2006; Oas等人,2014; Weitkunat等人,2014; Shwartz等人,2016) ...

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