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Zinc acetate dihydrate

公司名称: EMD Millipore
产品编号: 108802
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The Sulfur Oxygenase Reductase Activity Assay: Catalyzing a Reaction with Elemental Sulfur as Substrate at High Temperatures
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2017-07-20
[Abstract]  The sulfur oxygenase reductase (SOR) reaction is a dioxygen-dependent disproportionation of elemental sulfur (S0), catalyzed at optimal temperatures between 65 °C and 85 °C. Thiosulfate and sulfite are formed as oxidized products as well hydrogen sulfide as reduced product. External co-factors are not required. Usually, the SOR assay is performed in a milliliter scale in S0-containing Tris-buffer at high temperatures followed by colorimetric product quantification. In order to make the SOR assay more sensitive and better reproducible, several modifications were ... [摘要]  硫加氧酶还原酶(SOR)反应是元素硫的二氧依赖性歧化(S 0 ),在65℃至85℃之间的最佳温度下催化。 硫代硫酸盐和亚硫酸盐作为还原产物形成氧化产物以及硫化氢。 不需要外部辅助因素。 通常,SOR测定在高温下以含有S 0含有Tris缓冲液的毫升标度进行,随后进行比色产物定量。 为了使SOR测定更灵敏和更好的再现性,与原始SOR测定相比,实施了几个修改(Kletzin,1989)。 在这里,我们提供修饰的SOR测定和以下反应产物的定量。
【背景】硫氧化酶还原酶(SOR)催化与亚硫酸盐,硫代硫酸盐和硫化氢作为可检测产物的元素硫的二氧依赖性歧化。最初发现的SOR来自超嗜热,硫氧化古菌和细菌。报道的温度最佳值在65℃和85℃之间,pH值在pH5和pH7.4之间是最佳的(Emmel等人,1986; Kletzin,1989; Sun等人, ,2003; Pelletier等人,2008)。令人惊讶的是,源自嗜温细菌的新生儿烟草属(SOUTH)等(2012)的嗜碱性细菌和碱性嗜碱性硫代弧菌悖论的SORs(Rühlet al。还有在pH8.4和pH9.0下分别具有约80℃的温度最佳值。大多数SOR可以在E中生产。大肠杆菌通过异源基因表达,并且只有该酶的第一个描述源自从其天然来源纯化的蛋白质(Emmel等人,1986; Kletzin,1989; Pelletier et al。 ...

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