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Author:
Date:
2016-09-05
[Abstract] Leaf epidermal cell size and number are positively correlated to leaf area. Stomata are specialized epidermal cells vital for gas exchange and water transpiration. So, observation and statistical analysis of the leaf epidermal cells are valuable for the study of leaf development and response to environmental stimulus. The classical method is using the scanning electron microscope (SEM), which is an expensive and time-consuming method, thus makes the large-scale screening of epidermis impractical. Here we provide simple but effective methods (agarose-based epidermal imprinting and tape-based ...
[摘要] 叶表皮细胞大小和数量与叶面积正相关。 气孔是对气体交换和水蒸腾至关重要的专门的表皮细胞。 因此，叶表皮细胞的观察和统计分析对于叶发育和对环境刺激的反应的研究是有价值的。 经典的方法是使用扫描电子显微镜（SEM），这是一种昂贵和耗时的方法，因此使得大规模筛选表皮不切实际。 在这里，我们提供简单但有效的方法（基于琼脂糖的表皮印记和基于胶带的表皮撕裂），以解决这个问题，而不使用SEM。
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